MED17 antibody (Mediator Complex Subunit 17) (N-Term)

Details for Product anti-MED17 Antibody No. ABIN2778320, Supplier: Log in to see
Antigen
  • crsp6
  • CRSP6
  • CRSP77
  • DRIP80
  • TRAP80
  • 77kDa
  • C330002H14Rik
  • Crsp6
  • Trap80
  • mediator of RNA polymerase II transcription subunit 17
  • Mediator of RNA polymerase II transcription subunit 17
  • mediator complex subunit 17
  • AOR_1_2446154
  • VDBG_01663
  • med17
  • MED17
  • Med17
Alternatives
anti-Cow (Bovine) MED17 antibody for Western Blotting
Epitope
N-Term
15
10
9
8
4
4
3
2
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
79
29
21
2
1
1
1
1
1
1
1
1
Host
Rabbit
60
19
Clonality
Polyclonal
Conjugate
This MED17 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (IHC), Western Blotting (WB)
61
36
13
12
12
9
8
3
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CRSP6
Blocking Peptide Blocking peptide for this product available: ABIN975330
Sequence AAQILLKGAE RLTKSVTENQ ENKLQRDFNS ELLRLRQHWK LRKVGDKILG
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against CRSP6. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name CRSP6 (MED17 Antibody Abstract)
Background The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by CRSP6 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Alias Symbols: CRSP6, CRSP77, DRIP80, TRAP80
Protein Interaction Partner: UBC, BRCA1, CDK19, CDK8, MED26, POLR2C, ESR2, MED19, FBXW7, EPAS1, TFIP11, MED24, MED21, MED14, MED11, MED28, WDR33, CPSF2, UBD, BCL6, SMARCA4, SUZ12, MED10, CTDP1, SREBF1, MED25, MED15, TRA, MED1, OBFC1, MED18, ZC3H13, MED12, QKI, TRIP4, TADA2A, SUPT7L,
Protein Size: 651
Molecular Weight 73 kDa
Gene ID 9440
NCBI Accession NM_004268, NP_004259
UniProt Q9NVC6
Pathways Intracellular Steroid Hormone Receptor Signaling Pathway, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 651 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-MED17 antibody (Mediator Complex Subunit 17) (N-Term) (ABIN2778320) WB Suggested Anti-CRSP6 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Immunohistochemistry (IHC) image for anti-MED17 antibody (Mediator Complex Subunit 17) (N-Term) (ABIN2778320) anti-Mediator Complex Subunit 17 (MED17) (N-Term) antibody (Image 2)
Chromatin Immunoprecipitation (ChIP) image for anti-MED17 antibody (Mediator Complex Subunit 17) (N-Term) (ABIN2778320) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-MED17 antibody (Mediator Complex Subunit 17) (N-Term) (ABIN2778320) Rabbit Anti-MED17 Antibody ,Paraffin Embedded Tissue: Human neural cell
Cellul...
Background publications Wang, Sharma, Ren, Fondell: "A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression." in: The Journal of biological chemistry, Vol. 277, Issue 45, pp. 42852-8, 2002 (PubMed).