PRMT2 antibody (Protein Arginine Methyltransferase 2) (N-Term)

Details for Product anti-PRMT2 Antibody No. ABIN2778662, Supplier: Log in to see
Antigen
  • PRMT2
  • DKFZp459N1919
  • HRMT1L1
  • Hrmt1l1
  • AI504737
  • protein arginine methyltransferase 2
  • protein arginine N-methyltransferase 2
  • PRMT2
  • LOC100356731
  • Prmt2
  • prmt2
Alternatives
anti-Rat (Rattus) PRMT2 antibody for Chromatin Immunoprecipitation
Epitope
N-Term
27
20
6
6
6
3
3
3
2
2
1
1
1
1
Reactivity
Human, Rat (Rattus)
127
17
16
6
4
4
3
3
2
2
2
1
1
Host
Rabbit
81
33
13
Clonality
Polyclonal
Conjugate
This PRMT2 antibody is un-conjugated
6
5
5
5
5
5
1
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
111
50
33
32
13
10
3
2
2
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PRMT2
Blocking Peptide Blocking peptide for this product available: ABIN982232
Sequence ERAGCCGYIP ANHVGKHVDE YDPEDTWQDE EYFGSYGTLK LHLEMLADQP
Predicted Reactivity Human: 100%, Rat: 79%
Characteristics This is a rabbit polyclonal antibody against PRMT2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name PRMT2 (PRMT2 Antibody Abstract)
Background The protein arginine methyltransferases (PRMTs) include a family of proteins with related putative methyltransferase domains that modify chromatin and regulate cellular transcription. PRMT2 inhibits NF-kappaB-dependent transcription and promotes apoptosis and it exerts this effect by blocking nuclear export of IkappaB-alpha through a leptomycin-sensitive pathway, increasing nuclear IkappaB-alpha and decreasing NF-kappaB DNA binding. PRMT2 also rendered cells susceptible to apoptosis by cytokines or cytotoxic drugs, likely due to its effects on NF-kappaB.
Alias Symbols: HRMT1L1, MGC111373
Protein Interaction Partner: FIP1L1, SPAG8, RIPK2, BAG6, RB1, HNRNPA1, E2F1, UBC, PSMB3, HHV8GK18_gp81, CPSF7, DMRTB1, HNRNPUL1, PRMT2, THRB, PGR, NCOA6, NCOA1, ESR2, RXRA, PRRC2A, ESR1, PPARG, RARA,
Protein Size: 433
Molecular Weight 49 kDa
Gene ID 3275
NCBI Accession NM_001535, NP_001526
UniProt P55345
Research Area Chromatin and Nuclear Signaling
Pathways Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 433 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-PRMT2 antibody (Protein Arginine Methyltransferase 2) (N-Term) (ABIN2778662) WB Suggested Anti-PRMT2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Chromatin Immunoprecipitation (ChIP) image for anti-PRMT2 antibody (Protein Arginine Methyltransferase 2) (N-Term) (ABIN2778662) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...
Product cited in: Zhong, Cao, Liu, Liu, Wang, Liu, Chen, Yang, Zhang, Wu, Ding, Hong, Xiao, Zu, Wen: "Nuclear loss of protein arginine N-methyltransferase 2 in breast carcinoma is associated with tumor grade and overexpression of cyclin D1 protein." in: Oncogene, Vol. 33, Issue 48, pp. 5546-58, 2014 (PubMed).

Meyer, Wolf, Obendorf: "PRMT2, a member of the protein arginine methyltransferase family, is a coactivator of the androgen receptor." in: The Journal of steroid biochemistry and molecular biology, Vol. 107, Issue 1-2, pp. 1-14, 2007 (PubMed).