PRMT2 antibody (N-Term)

Catalog No. ABIN2778663

Cited in 4+ publications. The Rabbit Polyclonal anti-PRMT2 antibody (ABIN2778663) specifically detects PRMT2 in WB and IHC. The antibody is reactive with Human, Rat, Mouse, Cow, Horse, Pig, Guinea Pig, Dog and Rabbit samples.

Quick Overview for PRMT2 antibody (N-Term) (ABIN2778663)

Target: PRMT2 (Protein Arginine Methyltransferase 2 (PRMT2))

Reactivity: Human, Rat, Mouse, Cow, Horse, Pig, Guinea Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRMT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PRMT2 Antibody

Binding Specificity: N-Term

Purpose: PRMT2 Antibody - N-terminal region

Sequence: ESQGEEPAEC SEAGLLQEGV QPEEFVAIAD YAATDETQLS FLRGEKILIL

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PRMT2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRMT2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40196-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PRMT2 antibody (ABIN2778663)

Burton, Muller, Tu, Padilla-Longoria, Guccione, Torres-Padilla: "Single-cell profiling of epigenetic modifiers identifies PRDM14 as an inducer of cell fate in the mammalian embryo." in: Cell reports, Vol. 5, Issue 3, pp. 687-701, (2013) (PubMed).

Zhong, Cao, Zu, Hong, Yang, Liu, Xiao, Ding, Zhao, Liu, Wen: "Identification and characterization of novel spliced variants of PRMT2 in breast carcinoma." in: The FEBS journal, Vol. 279, Issue 2, pp. 316-35, (2012) (PubMed).

Spellman, Ahmed, Dubach, Gardiner: "Expression of trisomic proteins in Down syndrome model systems." in: Gene, Vol. 512, Issue 2, pp. 219-25, (2012) (PubMed).

Zhong, Cao, Hong, Yang, Zu, Xiao, Liu, Wen: "Identification and expression analysis of a novel transcript of the human PRMT2 gene resulted from alternative polyadenylation in breast cancer." in: Gene, Vol. 487, Issue 1, pp. 1-9, (2011) (PubMed).

Target Details for PRMT2

Target: PRMT2 (Protein Arginine Methyltransferase 2 (PRMT2))

Alternative Name: PRMT2

Background:

Background Information: The protein arginine methyltransferases (PRMTs) include a family of proteins with related putative methyltransferase domains that modify chromatin and regulate cellular transcription. PRMT2 inhibits NF-kappaB-dependent transcription and promotes apoptosis and it exerts this effect by blocking nuclear export of IkappaB-alpha through a leptomycin-sensitive pathway, increasing nuclear IkappaB-alpha and decreasing NF-kappaB DNA binding. PRMT2 also rendered cells susceptible to apoptosis by cytokines or cytotoxic drugs, likely due to its effects on NF-kappaB.

Gene Name: Protein arginine methyltransferase 2

Alternative Symbols: HRMT1L1

Protein Name: Protein arginine N-methyltransferase 2

Molecular Weight: 48kDa

Gene ID: 3275

NCBI Accession: NP_001526

UniProt: P55345

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding