SPI1 antibody (N-Term)

Catalog No. ABIN2779967

Product Details anti-SPI1 Antibody

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Binding Specificity: N-Term

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPI1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MLQACKMEGF SLTAPPSDDL VTYDSELYQR PMHDYYSFVG SDGESHSDHY

Predicted Reactivity: Mouse: 100%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against Sfpi1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N-terminal region of Mouse Sfpi1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 272 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Alternative Name: Sfpi1 (SPI1 Products)

Synonyms: OF antibody, PU.1 antibody, SFPI1 antibody, SPI-1 antibody, SPI-A antibody, Dis-1 antibody, Dis1 antibody, Sfpi-1 antibody, Sfpi1 antibody, Spi-1 antibody, Tcfpu1 antibody, Tfpu.1 antibody, Pu.1 antibody, cb1086 antibody, pu.1 antibody, pu1 antibody, sfpi1 antibody, si:by184l24.2 antibody, spi1 antibody, SPI1/PU.1 antibody, spi1l antibody, zgc:152991 antibody, Spi-1 proto-oncogene antibody, spleen focus forming virus (SFFV) proviral integration oncogene antibody, Spi-1 proto-oncogene b antibody, Spi-1 proto-oncogene a antibody, SPI1 antibody, Spi1 antibody, spi1b antibody, spi1a antibody

Background: Sfpi1 binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing.
Alias Symbols: Dis-1, Dis1, PU.1, Sfpi-1, Spi-1, Spi1, Tcfpu1, Tfpu.1, Sfpi1
Protein Interaction Partner: Prdm1, Jun, Hoxa10, Ep300, Kat6a, Irf4, Pml, Trim33, Cbfa2t3, Sin3a, Hdac2, Id2, Gata1, Emx1, Crebbp, Gata3, Csf1r, Il7r,
Protein Size: 272

Molecular Weight: 31 kDa

Gene ID: 20375

NCBI Accession: NM_011355, NP_035485

UniProt: P17433

Pathways: Stem Cell Maintenance