SMAD2 antibody (N-Term)
-
- Target See all SMAD2 Antibodies
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
-
Binding Specificity
- N-Term
-
Reactivity
- Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Sheep, Dog, Horse, Guinea Pig
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This SMAD2 antibody is un-conjugated
-
Application
- Western Blotting (WB)
- Sequence
- VVKRLLGWKK SAGGSGGAGG GEQNGQEEKW CEKAVKSLVK KLKKTGRLDE
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%
- Characteristics
- This is a rabbit polyclonal antibody against SMAD2. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Protein A purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human SMAD2
- Top Product
- Discover our top product SMAD2 Primary Antibody
-
-
- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
-
Antigen size: 467 AA
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
-
- Target
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
- Alternative Name
- SMAD2 (SMAD2 Products)
- Synonyms
- JV18 antibody, JV18-1 antibody, MADH2 antibody, MADR2 antibody, hMAD-2 antibody, hSMAD2 antibody, jv18 antibody, Xmad2 antibody, madh2 antibody, madr2 antibody, XSmad2 antibody, hmad-2 antibody, hsmad2 antibody, jv18-1 antibody, smad-2 antibody, fj43c06 antibody, wu:fj43c06 antibody, 7120426M23Rik antibody, Madh2 antibody, Madr2 antibody, Smad-2 antibody, mMad2 antibody, MAD homolog 2 antibody, Mothers against DPP homolog 2 antibody, SMAD 2 antibody, Smad2 antibody, SMAD family member 2 antibody, SMAD family member 2-Z antibody, SMAD family member 2 S homeolog antibody, SMAD2 antibody, SMAD2Z antibody, smad2 antibody, smad2.S antibody, Smad2 antibody
- Background
-
SMAD2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, SMAD2 is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin.The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
Alias Symbols: JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2
Protein Interaction Partner: HDAC2, UBC, SMAD4, SKIL, ANP32B, TUBA1B, SYT1, SOD1, ANP32E, PTMS, NEFM, JUN, ATP5I, RHOA, OTUB1, ZFYVE9, PPARG, TP53, TGFBR1, TRIM33, HCVgp1, TSC2, FHL3, FHL2, FHL1, CSNK1D, KLF5, SH2D2A, SKI, LCK, IRF7, IRF3, RNF111, LEMD3, PIK3CA, ST13, ST5, SRI, SPTBN
Protein Size: 467 - Molecular Weight
- 51 kDa
- Gene ID
- 4087
- NCBI Accession
- NM_005901, NP_005892
- UniProt
- Q15796
- Pathways
- Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
-