BRD4 antibody (Bromodomain Containing 4) (C-Term)

Details for Product anti-BRD4 Antibody No. ABIN2780921, Supplier: Log in to see
Antigen
  • brd4-b
  • BRD4
  • fb18g03
  • wu:fb18g03
  • CAP
  • HUNK1
  • HUNKI
  • MCAP
  • Brd5
  • WI-11513
  • bromodomain containing 4
  • brd4
  • BRD4
  • Brd4
Alternatives
anti-Human BRD4 antibody for Western Blotting
Epitope
C-Term
23
17
17
15
11
8
8
3
3
2
2
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
106
38
20
7
6
6
6
5
4
2
1
1
Host
Rabbit
101
5
1
Clonality
Polyclonal
Conjugate
This BRD4 antibody is un-conjugated
7
7
6
6
6
6
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunofluorescence (IF), Western Blotting (WB)
74
50
48
35
27
24
5
4
3
2
1
Options
Supplier
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Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human BRD4
Blocking Peptide Blocking peptide for this product available: ABIN973879
Sequence EIEIDFETLK PSTLRELERY VTSCLRKKRK PQAEKVDVIA GSSKMKGFSS
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against BRD4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name BRD4 (BRD4 Antibody Abstract)
Background BRD4 is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting.The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15,19)(q13,p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described.
Alias Symbols: CAP, HUNKI, MCAP, HUNK1
Protein Interaction Partner: AFF1, CDK9, CCNT1, STAT3, JMJD6, TCERG1, HEXIM1, RN7SK, PRPF40A, vif, RPL6, MYC, MLLT1, ELAVL1, SUMO2, UBC, YWHAZ, KAT8, C8orf33, MESDC2, HIST1H4A, HIST1H3A, YWHAE, MED1, EP300, KDM5B, C7orf25, CHFR, CLDN1, RFC1, RFC4, HIST2H3C, HIST2H4A, MED12, MED24, ME
Protein Size: 722
Molecular Weight 80 kDa
Gene ID 23476
NCBI Accession NM_014299, NP_055114
Pathways Chromatin Binding
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 722 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Immunofluorescence (IF) image for anti-Bromodomain Containing 4 (BRD4) (C-Term) antibody (ABIN2780921) Sample Type: HeLa
Primary Antibody Dilution: 4 µg/mL
Secondary Antibody: ...
Chromatin Immunoprecipitation (ChIP) image for anti-Bromodomain Containing 4 (BRD4) (C-Term) antibody (ABIN2780921) Chromatin Immunoprecipitation (ChIP) Using BRD4 Antibody - C-terminal region (ARP3907...
Background publications Schweiger, You, Howley: "Bromodomain protein 4 mediates the papillomavirus E2 transcriptional activation function." in: Journal of virology, Vol. 80, Issue 9, pp. 4276-85, 2006 (PubMed).

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