PRIM1 antibody (N-Term)
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- Target See all PRIM1 Antibodies
- PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Cow, Rabbit, Guinea Pig, Horse, Dog, Zebrafish (Danio rerio), Saccharomyces cerevisiae
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PRIM1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- SQYYRWLNYG GVIKNYFQHR EFSFTLKDDI YIRYQSFNNQ SDLEKEMQKM
- Predicted Reactivity
- Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 80%, Zebrafish: 79%
- Characteristics
- This is a rabbit polyclonal antibody against PRIM1. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Protein A purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human PRIM1
- Top Product
- Discover our top product PRIM1 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 420 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- PRIM1 (Primase, DNA, Polypeptide 1 (49kDa) (PRIM1))
- Alternative Name
- PRIM1 (PRIM1 Products)
- Synonyms
- AI324982 antibody, p49 antibody, dnapol-alpha50 antibody, DNA primase, p49 subunit antibody, DNA primase subunit 1 antibody, DNA primase subunit 1 S homeolog antibody, Prim1 antibody, PRIM1 antibody, prim1.S antibody
- Background
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The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.
Alias Symbols: MGC12308, p49
Protein Interaction Partner: FAM96B, CIAO1, UBC, MMS19, MDC1, POLA2, PRIM2, POLE, POLA1, COPS6, RPA3, RPA2, RPA1, RAE1, MRPL38, TP53, XRCC5,
Protein Size: 420 - Molecular Weight
- 50 kDa
- Gene ID
- 5557
- NCBI Accession
- NM_000946, NP_000937
- UniProt
- P49642
- Pathways
- Telomere Maintenance, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, SARS-CoV-2 Protein Interactome
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