Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Middle Region) antibody

Details for Product No. ABIN2785912, Supplier: Log in to see
Antigen
  • APPL
  • DIP13alpha
  • 2900057D21Rik
  • 7330406P05Rik
  • AI585782
  • AW209077
  • BB022931
  • C88264
  • DIP13
  • RGD1309388
  • adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
  • APPL1
  • Appl1
Epitope
Middle Region
15
9
5
5
4
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
64
32
20
6
4
3
3
2
2
2
1
1
Host
Rabbit
43
20
3
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Application
Western Blotting (WB)
60
33
17
12
3
3
1
1
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human APPL1
Blocking Peptide Blocking peptide for this product available: ABIN973345
Sequence GQAKAFGQGG RRTNPFGESG GSTKSETEDS ILHQLFIVRF LGSMEVKSDD
Predicted Reactivity Cow: 86%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 75%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against APPL1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name APPL1 (APPL1 Antibody Abstract)
Background The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A
Alias Symbols: APPL, DIP13alpha
Protein Interaction Partner: RHEBL1, UBE2O, APPL2, APPL1, FARS2, MEOX1, MAGEA9, UBC, KIAA1468, CLUH, TTC4, TRAF6, ADIPOR1, HDAC5, HDAC3, HDAC2, PIK3R1, AR, AKT1, CMTM4, PLEKHF2, KXD1, DPYSL5, EGFR, RAB21, VCP, RAB5A, AKT2, ADIPOR2, FSHR, ELAVL1, MTA2, RBBP7, RUVBL2, RBBP4, HDAC1, TRA
Protein Size: 709
Molecular Weight 80 kDa
Gene ID 26060
NCBI Accession NM_012096, NP_036228
UniProt Q9UKG1
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 709 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Middle Region) antibody (ABIN2785912) WB Suggested Anti-APPL1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive...
Background publications McCrea, Paradise, Tomasini, Addis, Melis, De Matteis, De Camilli: "All known patient mutations in the ASH-RhoGAP domains of OCRL affect targeting and APPL1 binding." in: Biochemical and biophysical research communications, Vol. 369, Issue 2, pp. 493-9, 2008 (PubMed).