NAE1 antibody (C-Term)

Catalog No. ABIN2788045

Product Details anti-NAE1 Antibody

Target: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Horse, Pig, Rabbit, Zebrafish (Danio rerio), Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAE1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ARALKEFVAK EGQGNLPVRG TIPDMIADSN KYIKLQNVYR EKAKKDAAAV

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against Nae1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Mouse Nae1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 534 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NAE1

Target: NAE1 (NEDD8 Activating Enzyme E1 Subunit 1 (NAE1))

Alternative Name: Nae1 (NAE1 Products)

Synonyms: APPBP1 antibody, CG7828 antibody, Dmel\\CG7828 antibody, anon-WO0140519.225 antibody, dAPP-BP1 antibody, dAPPBP1 antibody, A-116A10.1 antibody, ula-1 antibody, Appbp1 antibody, 59kDa antibody, appbp1 antibody, zgc:66148 antibody, beta-Amyloid precursor protein binding protein 1 antibody, NEDD8 activating enzyme E1 subunit 1 antibody, NEDD8 activating enzyme E1 subunit 1 S homeolog antibody, nedd8 activating enzyme E1 subunit 1 antibody, NEDD8-activating enzyme E1 regulatory subunit antibody, APP-BP1 antibody, NAE1 antibody, Nae1 antibody, nae1.S antibody, nae1 antibody, ula-1 antibody

Background: Nae1 is a regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.
Alias Symbols: 59 kDa, Appbp1
Protein Size: 534

Molecular Weight: 60 kDa

Gene ID: 234664

NCBI Accession: NM_144931, NP_659180

UniProt: Q8VBW6