MRPL12 antibody (Middle Region)

Catalog No. ABIN2790270

Product Details anti-MRPL12 Antibody

Target: MRPL12 (Mitochondrial Ribosomal Protein L12 (MRPL12))

Binding Specificity: Middle Region

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL12 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KKTLKIQDVG LVPMGGVMSG AVPAAAAQEA VEEDIPIAKE RTHFTVRLTE

Predicted Reactivity: Dog: 77%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against MRPL12. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of Human MRPL12

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 198 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MRPL12

Target: MRPL12 (Mitochondrial Ribosomal Protein L12 (MRPL12))

Alternative Name: MRPL12 (MRPL12 Products)

Synonyms: 5c5-2 antibody, L12mt antibody, MRP-L31/34 antibody, MRPL7 antibody, MRPL7/L12 antibody, RPML12 antibody, GB14723 antibody, MRPL12 antibody, 0610034O11Rik antibody, 1500031N16Rik antibody, MRP-L12 antibody, Rpml12 antibody, Rpm12 antibody, im:6893415 antibody, zgc:112445 antibody, mrpl12 antibody, mitochondrial ribosomal protein L12 antibody, 39S ribosomal protein L12, mitochondrial antibody, mitochondrial nucleoid protein antibody, mitochondrial ribosomal protein L12 L homeolog antibody, MRPL12 antibody, LOC409926 antibody, CAALFM_C207190CA antibody, Mrpl12 antibody, mrpl12 antibody, mrpl12.L antibody

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
Alias Symbols: 5c5-2, FLJ60124, L12mt, MGC8610, MRP-L31/34, MRPL7, MRPL7/L12, RPML12
Protein Interaction Partner: MDM2, PARK2, TARDBP, MRPS27, ICT1, mug82, ADRB2, NPM1, SOX2, OLIG1, POLR1D, UBC, RARG, COX6A2, C1QBP, RPL18, RPL12, RPL11, RPL7A, RBM3, ILF3, HNRNPD, AK2, ACOX1, TMEM9, MRPL10, MRPL52, GADD45GIP1, EPPK1, MRPL1, MRPL9, MRPL11, MRPL41, MRPS9, MRPS26, NIT2,
Protein Size: 198

Molecular Weight: 21 kDa

Gene ID: 6182

NCBI Accession: NM_002949, NP_002940

UniProt: P52815