Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from the C-terminal region of human PACSIN2.
PACSIN2
Reactivity: Human
WB
Host: Mouse
Polyclonal
unconjugated
Application Notes
WB: 1:1000. IHC-P: 1:25. IHC-P: 1:50~100
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date
6 months
Copeland, Han, Tiwari, Austin, Loyd, West, Kenworthy: "A disease-associated frameshift mutation in caveolin-1 disrupts caveolae formation and function through introduction of a de novo ER retention signal." in: Molecular biology of the cell, Vol. 28, Issue 22, pp. 3095-3111, (2018) (PubMed).
Han, Copeland, Kawano, Rosenzweig, Austin, Shahmirzadi, Tang, Raghunathan, Chung, Kenworthy: "Characterization of a caveolin-1 mutation associated with both pulmonary arterial hypertension and congenital generalized lipodystrophy." in: Traffic (Copenhagen, Denmark), Vol. 17, Issue 12, pp. 1297-1312, (2017) (PubMed).
Bahl, Xie, Spagnol, Sorgen, Naslavsky, Caplan et al.: "EHD3 Protein Is Required for Tubular Recycling Endosome Stabilization, and an Asparagine-Glutamic Acid Residue Pair within Its Eps15 Homology (EH) Domain Dictates Its Selective Binding to NPF ..." in: The Journal of biological chemistry, Vol. 291, Issue 26, pp. 13465-78, (2016) (PubMed).
Cai, Giridharan, Zhang, Saxena, Bahl, Schmidt, Sorgen, Guo, Naslavsky, Caplan: "Differential roles of C-terminal Eps15 homology domain proteins as vesiculators and tubulators of recycling endosomes." in: The Journal of biological chemistry, Vol. 288, Issue 42, pp. 30172-80, (2013) (PubMed).
Giridharan, Cai, Vitale, Naslavsky, Caplan: "Cooperation of MICAL-L1, syndapin2, and phosphatidic acid in tubular recycling endosome biogenesis." in: Molecular biology of the cell, Vol. 24, Issue 11, pp. 1776-90, S1-15, (2013) (PubMed).
de Kreuk, Anthony, Geerts, Hordijk: "The F-BAR protein PACSIN2 regulates epidermal growth factor receptor internalization." in: The Journal of biological chemistry, Vol. 287, Issue 52, pp. 43438-53, (2012) (PubMed).
Hansen, Howard, Nichols: "Pacsin 2 is recruited to caveolae and functions in caveolar biogenesis." in: Journal of cell science, Vol. 124, Issue Pt 16, pp. 2777-85, (2011) (PubMed).
de Kreuk, Nethe, Fernandez-Borja, Anthony, Hensbergen, Deelder, Plomann, Hordijk: "The F-BAR domain protein PACSIN2 associates with Rac1 and regulates cell spreading and migration." in: Journal of cell science, Vol. 124, Issue Pt 14, pp. 2375-88, (2011) (PubMed).
Target
PACSIN2
(Protein Kinase C and Casein Kinase Substrate in Neurons 2 (PACSIN2))
zgc:77519 antibody, PACSIN2 antibody, DKFZp459J1025 antibody, SDPII antibody, AI197433 antibody, sdpii antibody, SdpII antibody, protein kinase C and casein kinase substrate in neurons 2 antibody, protein kinase C and casein kinase substrate in neurons 2 S homeolog antibody, pacsin2 antibody, PACSIN2 antibody, Pacsin2 antibody, pacsin2.S antibody
Background
PACSIN may play a role in vesicle formation and transport. This protein homo- and hetero-aggregates with other PACSINs. It also binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). The protein exhibits a cvesicle-like cytoplasmic distribution and is ubiquitously expressed. PACSIN is phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). The protein contains 1 FCH domain and 1 SH3 domain.