Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This PACSIN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PACSIN3.
Pacsin1 antibody, wu:fa11d03 antibody, wu:fj67e09 antibody, wu:fq16a06 antibody, zgc:56324 antibody, MGC82145 antibody, PACSIN3 antibody, sdpiii antibody, SDPIII antibody, 4921507A02Rik antibody, 6330413E15Rik antibody, AW413130 antibody, SdpIII antibody, protein kinase C and casein kinase substrate in neurons 3 antibody, protein kinase C and casein kinase substrate in neurons 3 L homeolog antibody, pacsin3 antibody, PACSIN3 antibody, pacsin3.L antibody, Pacsin3 antibody
Background
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.