This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen
This CYP27B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-508 amino acids from the C-terminal region of human CYP27B1.
CYP27B1
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
HRP
Application Notes
WB: 1:500. WB: 1:2000. IHC-P: 1:10~50. FC: 1:25
Restrictions
For Research Use only
Format
Liquid
Buffer
Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid freeze-thaw cycles.
Storage
4 °C,-20 °C
Storage Comment
Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date
6 months
Chiang, Yeh, Huang, Yeh, S Pang, Hsu, Chen, Kuo, Kittaka, Chen, Juang: "25(OH)D Is Effective to Repress Human Cholangiocarcinoma Cell Growth through the Conversion of 25(OH)D to 1α,25(OH)₂D₃." in: International journal of molecular sciences, Vol. 17, Issue 8, (2016) (PubMed).
Schlosser, Carroll, Soler, Pasquini, Mulligan: "Reduced sinonasal levels of 1?-hydroxylase are associated with worse quality of life in chronic rhinosinusitis with nasal polyps." in: International forum of allergy & rhinology, (2015) (PubMed).
Mandell, Seedorf, Ryan, Gien, Cramer, Abman: "Antenatal endotoxin disrupts lung vitamin D receptor and 25-hydroxyvitamin D 1?-hydroxylase expression in the developing rat." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 309, Issue 9, pp. L1018-26, (2015) (PubMed).
Mulligan, Nagel, OConnell, Wentzel, Atkinson, Schlosser: "Cigarette smoke exposure is associated with vitamin D3 deficiencies in patients with chronic rhinosinusitis." in: The Journal of allergy and clinical immunology, Vol. 134, Issue 2, pp. 342-349.e1, (2014) (PubMed).
Target
CYP27B1
(Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))
CYP27B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.