AIF1 antibody (Allograft Inflammatory Factor 1) (C-Term)

Details for Product anti-AIF1 Antibody No. ABIN2857032, Supplier: Log in to see
Antigen
  • fa04b11
  • wu:fa04b11
  • AIF1
  • aif1
  • AIF-1
  • IBA1
  • IRT-1
  • IRT1
  • Bart1
  • iba1
  • mrf-1
  • AI607846
  • D17H6S50E
  • G1
  • Iba1
  • AIF
  • AIF1L
  • allograft inflammatory factor 1
  • Allograft inflammatory factor 1
  • allograft inflammatory factor 1-like
  • aif1
  • AIF1
  • Aif1
  • LOC100350092
  • C9ORF58
Alternatives
anti-Human AIF1 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
C-Term
36
25
20
15
14
14
10
8
8
6
4
4
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
207
105
96
8
7
2
2
1
1
Host
Rabbit
139
56
34
2
1
Clonality
Polyclonal
Conjugate
This AIF1 antibody is un-conjugated
23
22
11
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
182
123
103
65
28
27
19
13
10
10
7
4
2
2
2
2
1
Options
Supplier
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Supplier Product No.
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'Independent Validation' Badge
Antigen AIF1
Lot Number 39476
Method validated Immunofluorescence
Positive Control PFA fixed tissue section brain of CX3CR1 GFP mouse. Staining with Iba1 (Wako, 019-19741) and Donkey anti-rabbit Fab’2 AlexaFluor 647
Notes Passed. Immunostaining of mouse brain samples with ABIN2857032 shows specifically the expected staining pattern.
Primary Antibody ABIN2857032
Secondary Antibody donkey Fab’2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806)
Protocol
  • Harvest Brain from 13 weeks old mice in 1x Dulbecco’s phosphate buffered saline (DPBS) (Gibco Life Technologies, 14200-067).
  • Fix mouse brain in antigen fix (Diapath, P0014) for 2h at RT.
  • Wash tissue in 0.1M pH7.4 phosphate buffer at for 1h at 4°C.
  • Dehydrate tissue in 30% sucrose solution ON at 4°C.
  • Snap freeze tissue in Tissue Freezing Medium (ElectroMicroscopy Science, 72592-C) at -80°C.
  • Cut blocks into 20µm sections using a cryostat (Leica, CM3050 S).
  • Transfer sections to a slide.
  • Create a hydrophobic barrier on the slide around sections with Dako pen (Dako, S2002, lot 00081640).
  • Place slide in a humidified chamber and rehydrate sections in 0.1M TrisHCl pH7.4 for 10min at RT.
  • Gently remove buffer by tapping slide.
  • Permeabilize tissue in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA, for 20min at RT.
  • Incubate sections with primary AIF1 (c-term) (antibodies-online, ABIN2857032, Lot 39476) diluted 1:500 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 5h at RT. Incubate the negative contrls sections with diluted rabbit serum.
  • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
  • Incubate sections with secondary donkey Fab’2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806) diluted 1:500 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 and 0.5% BSA ON at RT.
  • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
  • Add approximately 10μl of Slowfade® Gold antifade reagent (Life technologies, 536937, lot 1226836) for each section and mount cover slip.
  • Image acquisition on a LSM 880 (Zeiss), 20x magnification, 1000 resolution.
Experimental Notes Microglial cells in the brain of the CX3CR1 GFP mouse expressing GFP are known to express Aif-1. Immunostaining of the PFA fixed tissue sections with ABIN2857032 showed the expected co-localization of the GFP and Aif-1 signals.
Validation Images
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) Colocalization (C) of the signals in PFA fixed tissue sections of CX3CR1 GFP mouse br...
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.
Isotype IgG
Cross-Reactivity Cow (Bovine), Pig (Porcine), Rhesus Monkey
Cross-Reactivity (Details) Pig (100 %), Rhesus Monkey (100 %), Bovine (92 %)
Characteristics Rabbit Polyclonal antibody to Iba1 (allograft inflammatory factor 1)
Iba1 antibody
Purification Purified by antigen-affinity chromatography.
Alternative Name Iba1 (AIF1 Antibody Abstract)
Background This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.

Molecular Weight 17 kDa
Gene ID 199
Pathways Smooth Muscle Cell Migration
Application Notes Suggested dilution Reference Immunohistochemistry Assay-dependent dilution IHC (Frozen sections) Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceImmunohistochemistryAssay-dependent dilution IHC (Frozen sections)Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: Molt-4 , THP-1 , HL-60 , *Mouse brain

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody a...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using I...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohisto...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochem...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma ...
Western Blotting (WB) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) WB Image Iba1 antibody detects Iba1 protein by western blot analysis. A. 30 μg THP-1 ...
Western Blotting (WB) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) WB Image Iba1 antibody detects Iba1 protein by Western blot analysis. A. 50 µg mouse ...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032) IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemic...
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