CDKN1B antibody

Catalog No. ABIN290297

The Rabbit Polyclonal anti-CDKN1B antibody is suitable to detect CDKN1B in samples from Human. It has been validated for WB, ELISA and IP.

Quick Overview for CDKN1B antibody (ABIN290297)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-CDKN1B Antibody

Specificity: Antiserum will specifically react with a p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse. Reactivity against p27 from other species is unknown

Purification: Delipidated and defibrinated

Immunogen: Full length recombinant human p27 protein.

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:40000), IP, WB (1:1000 - 1:4000)

Usage: This antibody is suitable for use in ELISA, immunoprecipitation, coimmunoprecipitation and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kD in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. No reactivity is observed with p27 from mouse. This antibody is suitable for immunoprecipitation of in vitro translated protein and cell lysates (C212, 3T3-L1) and will co-precipitate associated proteins. Optimal titers for other applications should be determined by the researcher.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: CDKN1B / p27 Kip1

Background: Name/Gene ID: CDKN1B

Synonyms: CDKN1B, KIP1, MEN1B, P27 Kip1, p27KIP1, MEN4, CDKN4

Gene ID: 1027

UniProt: P46527

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy