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CD1a Molecule (CD1A) antibody (APC)

Details for Product No. ABIN301984, Supplier: Log in to see
Antigen
  • CD1A
  • CD1.1
  • CD1.2
  • CD1A2
  • CD1
  • FCB6
  • HTA1
  • R4
  • T6
  • CD1B
  • AI747460
  • Cd1a
  • Cd1d
  • Ly-38
  • CD1b molecule
  • CD1 antigen
  • CD1a molecule
  • CD1d1 antigen
  • CD1B
  • CD1.1
  • CD1A
  • Cd1d1
Reactivity
Human
509
29
25
15
8
6
4
Host
Mouse
395
118
Clonality (Clone)
Monoclonal ()
Conjugate
APC
42
36
26
14
14
12
11
10
10
10
9
9
9
9
9
7
7
7
3
3
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
363
172
166
158
135
120
107
107
38
15
4
3
3
3
1
1
1
1
Options
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Immunogen Human thymocytes
Clone HI149
Isotype IgG1
Specificity The antibody HI149 reacts with CD1a (T6), a 49 kDa polypeptide associated with beta2-microglobulin expressed on cortical thymocytes (strongly), Langerhans cells, dendritic cells and some T cell leukemias and lymphomas.
The antibody does not react with peripheral blood T and B lymphocytes, monocytes, granulocytes, platelets and erythrocytes.
HLDA V, WS Code CD01.01
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name CD1A (CD1A Antibody Abstract)
Background CD1a, together with CD1b and c, belongs to group 1 of CD1 glycoproteins. These proteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. Unlike CD1b, CD1a is excluded from late endosomal compartments and instead traffics independently in the recycling pathway of the early endocytic system, and CD1a antigen presentation is independent upon vesicular acidification.
Research Area Surface Receptors of Immune Cells, Adaptive Immunity, CD Antigens, Immunology
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Product cited in: Mayer, Irschick, Moser, Wurm, Huemer, Romani, Irschick: "Characterization of antigen-presenting cells in fresh and cultured human corneas using novel dendritic cell markers." in: Investigative ophthalmology & visual science, Vol. 48, Issue 10, pp. 4459-67, 2007 (PubMed).

Angel, Lala, Chen, Edgar, Ostrovsky, Dunbar: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007 (PubMed).

Perros, Dorfmüller, Souza, Durand-Gasselin, Mussot, Mazmanian, Hervé, Emilie, Simonneau, Humbert: "Dendritic cell recruitment in lesions of human and experimental pulmonary hypertension." in: The European respiratory journal, Vol. 29, Issue 3, pp. 462-8, 2007 (PubMed).

Demedts, Brusselle, Vermaelen, Pauwels: "Identification and characterization of human pulmonary dendritic cells." in: American journal of respiratory cell and molecular biology, Vol. 32, Issue 3, pp. 177-84, 2005 (PubMed).

Chen, Murakami, Oppenheim, Howard: "Triptolide, a constituent of immunosuppressive Chinese herbal medicine, is a potent suppressor of dendritic-cell maturation and trafficking." in: Blood, Vol. 106, Issue 7, pp. 2409-16, 2005 (PubMed).

Kirsch, Zeyda, Stuhlmeier, Grisar, Smolen, Watschinger, Stulnig, Hörl, Zlabinger, Säemann: "The active metabolite of leflunomide, A77 1726, interferes with dendritic cell function." in: Arthritis research & therapy, Vol. 7, Issue 3, pp. R694-703, 2005 (PubMed).

Hunger, Sieling, Ochoa, Sugaya, Burdick, Rea, Brennan, Belisle, Blauvelt, Porcelli, Modlin: "Langerhans cells utilize CD1a and langerin to efficiently present nonpeptide antigens to T cells." in: The Journal of clinical investigation, Vol. 113, Issue 5, pp. 701-8, 2004 (PubMed).

McKenna, Asplund, Kroft: "Immunophenotypic analysis of hematogones (B-lymphocyte precursors) and neoplastic lymphoblasts by 4-color flow cytometry." in: Leukemia & lymphoma, Vol. 45, Issue 2, pp. 277-85, 2004 (PubMed).

Säemann, Parolini, Böhmig, Kelemen, Krieger, Neumüller, Knarr, Kammlander, Hörl, Diakos, Stuhlmeier, Zlabinger: "Bacterial metabolite interference with maturation of human monocyte-derived dendritic cells." in: Journal of leukocyte biology, Vol. 71, Issue 2, pp. 238-46, 2002 (PubMed).

Background publications Favali, Tavares, Claruencio, Barral, Barral-Netto, Brodskyn: "Leishmania amazonensis infection impairs differentiation and function of human dendritic cells." in: Journal of leukocyte biology, Vol. 82, Issue 6, pp. 1401-6, 2007 (PubMed).

Raftery, Winau, Kaufmann, Schaible, Schönrich: "CD1 antigen presentation by human dendritic cells as a target for herpes simplex virus immune evasion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 9, pp. 6207-14, 2006 (PubMed).

Hiromatsu, Dascher, Sugita, Gingrich-Baker, Behar, LeClair, Brenner, Porcelli: "Characterization of guinea-pig group 1 CD1 proteins." in: Immunology, Vol. 106, Issue 2, pp. 159-72, 2002 (PubMed).

Sugita, Grant, van Donselaar, Hsu, Rogers, Peters, Brenner: "Separate pathways for antigen presentation by CD1 molecules." in: Immunity, Vol. 11, Issue 6, pp. 743-52, 2000 (PubMed).