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CD19 antibody (CD19 Molecule) (PerCP)

Details for Product anti-CD19 Antibody No. ABIN302053, Supplier: Log in to see
Antigen
  • AW495831
  • B4
  • CD19
  • CVID3
Alternatives
anti-Human CD19 antibody for Immunoprecipitation
Reactivity
Human
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This CD19 antibody is conjugated to PerCP
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Application
Flow Cytometry (FACS)
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Supplier
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Immunogen Daudi human Burkitt lymphoma cell line
Clone LT19
Isotype IgG1
Specificity The antibody LT19 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it is lost on plasma cells.
Characteristics The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Alternative Name CD19 (CD19 Antibody Abstract)
Background CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Flow Cytometry (FACS) image for anti-CD19 antibody (CD19 Molecule)  (PerCP) (ABIN302053) Surface staining of human peripheral blood cells with anti-human CD19 (LT19) APC.
Product cited in: Kayserova, Vcelakova, Stechova, Dudkova, Hromadkova, Sumnik, Kolouskova, Spisek, Sediva: "Decreased dendritic cell numbers but increased TLR9-mediated interferon-alpha production in first degree relatives of type 1 diabetes patients." in: Clinical immunology (Orlando, Fla.), Vol. 153, Issue 1, pp. 49-55, 2014

Elias, Flo, Lopez, Zorzopulos, Montaner, Rodriguez: "Strong cytosine-guanosine-independent immunostimulation in humans and other primates by synthetic oligodeoxynucleotides with PyNTTTTGT motifs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 7, pp. 3697-704, 2003

Background publications Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009

Gonçalves, Castro, Henriques, Oliveira, Pinheiro, Oliveira, Sreenu, Evans, Davis, Moreira, Carmo: "Molecular cloning and analysis of SSc5D, a new member of the scavenger receptor cysteine-rich superfamily." in: Molecular immunology, Vol. 46, Issue 13, pp. 2585-96, 2009

Rayment, Kooij, Zhang, Siebold, Murphy, Allen, Willcox, Roberts: "Evidence for the specificity for platelet HPA-1a alloepitope and the presenting HLA-DR52a of diverse antigen-specific helper T cell clones from alloimmunized mothers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 1, pp. 677-86, 2009

Shao, Suresh, Vakil, Gomer, Pilling: "Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation." in: Journal of leukocyte biology, Vol. 83, Issue 6, pp. 1323-33, 2008

Allen, Pang, Skowera, Ellis, Rackham, Lozanoska-Ochser, Tree, Leslie, Tremble, Dayan, Peakman: "Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture." in: Diabetes, Vol. 58, Issue 1, pp. 138-45, 2008

Barat, Gilbert, Imbeault, Tremblay: "Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4+ T lymphocytes." in: Retrovirology, Vol. 5, pp. 30, 2008

Harrison, Franklin, Campbell: "Enumeration of blood dendritic cells in patients with multiple myeloma at presentation and through therapy." in: Leukemia & lymphoma, Vol. 49, Issue 12, pp. 2272-83, 2008

Vantourout, Martinez, Fabre, Collet, Champagne: "Ecto-F1-ATPase and MHC-class I close association on cell membranes." in: Molecular immunology, Vol. 45, Issue 2, pp. 485-92, 2007

Shi, Xie, Chang, Zhou, Tedder, Mohan: "CD19 hyperexpression augments Sle1-induced humoral autoimmunity but not clinical nephritis." in: Arthritis and rheumatism, Vol. 56, Issue 9, pp. 3057-69, 2007

Inabe, Kurosaki: "Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement." in: Blood, Vol. 99, Issue 2, pp. 584-9, 2002

Fujimoto, Poe, Jansen, Sato, Tedder: "CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7088-94, 1999