HLA Class I Histocompatibility Antigen, alpha Chain E (HLAE) antibody

Antigen: HLA Class I Histocompatibility Antigen, alpha Chain E (HLAE)

Synonyms: MHC, QA1, EA1.2, EA2.1, HLA-6.2, DKFZp686P19218

Clonality: Monoclonal (MEM-E-08)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Catalog no.: ABIN302071

Additional Information

Alternative name: HLA-E

Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

Format: Purified

Isotype: IgG1

Clone: MEM-E-08

Specificity: The antibody MEM-E/08 recognized native surface-expressed HLA-E, but not denaturatedheavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib,nonclassical) and it is expressed on many types of the human cells.The published results revealed that antibody is remarkably specific for HLA-E, only withweak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia):HLA-A24, -B7, -B27, -B51, -B54, -C7.

Application Details

Application Notes: Flow Cytometry - Positive control: human cell line LCL 721. 221 (HLA-E transfected)

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Major Histocompatibility Complex (MHC), Immunology

Restrictions: For Research Use only

Publications

Publications: Allard, Oger, Vignard et al.: "Serum soluble HLA-E in melanoma: a new potential immune-related marker in cancer." in: PLoS ONE, Vol. 6, Issue 6, pp. e21118, 2011 (PubMed).