PCNA antibody (N-Term)

Catalog No. ABIN302209

This Mouse Monoclonal antibody specifically detects PCNA in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for PCNA antibody (N-Term) (ABIN302209)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: K1A3

Product Details anti-PCNA Antibody

Binding Specificity: AA 1-261, N-Term

Characteristics: Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human PCNA (1-261aa) purified from E. coli

Isotype: IgG3

Application Details

Application Notes: ELISA. Western blot: 1: 1,000 approx. 1: 3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) with 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighboring tumors.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy