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PCNA antibody (AA 1-261)

Name: PCNA antibody (AA 1-261)
SKU: ABIN562135
Price: 611.43 USD
Availability: InStock

PCNA Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 1G7 unconjugated

PCNA monoclonal antibody (M02), clone 1G7 Western Blot analysis of PCNA expression in Hela .

Immunofluorescence of monoclonal antibody to PCNA on HeLa cell.

Detection limit for recombinant GST tagged PCNA is approximately 0.

ELISA

Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M02), clone 1G7.

Immunoprecipitation of PCNA transfected lysate using anti-PCNA monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with PCNA MaxPab rabbit polyclonal antibody.

Western Blot detection against Immunogen (54.

7 images

(1 reference)

Catalog No. ABIN562135

Datasheet as PDF

Target See all PCNA Antibodies

PCNA (Proliferating Cell Nuclear Antigen (PCNA))
Binding Specificity
16

16

16

15

13

9

8

6

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-261
Reactivity
208

153

152

57

32

28

23

19

19

18

18

14

11

11

7

5

5

4

4

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1
Human
Host
135

86

3
Mouse
Compatible Secondaries
Clonality
134

89

1
Monoclonal
Conjugate
116

16

13

8

5

5

5

5

5

5

5

5

5

5

5

4

2

2

1

1

1

1

1

1

1

1
This PCNA antibody is un-conjugated
Application
194

68

65

59

59

49

48

40

30

16

15

11

5

4

3

2

2

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Purpose

Mouse monoclonal antibody raised against a full length recombinant PCNA.

Sequence

MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S

Cross-Reactivity

Human

Characteristics

Antibody Reactive Against Recombinant Protein.

Immunogen

PCNA (AAH00491, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Clone

1G7

Isotype

IgG2a

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Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Buffer

In 1x PBS, pH 7.4

Handling Advice

Aliquot to avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Mason, Logan, Budke, Wu, Pawlowski, Weichselbaum, Kozikowski, Bishop, Connell: "The RAD51-stimulatory compound RS-1 can exploit the RAD51 overexpression that exists in cancer cells and tumors." in: Cancer research, Vol. 74, Issue 13, pp. 3546-55, (2014) (PubMed).
Target

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name

PCNA (PCNA Products)

Synonyms

PCNAR antibody, Pcna/cyclin antibody, pcna antibody, AO090003000846 antibody, 53/13 antibody, CG9193 antibody, DmPCNA antibody, Dmel\\CG9193 antibody, MUS209 antibody, Mus209 antibody, PCNA antibody, PCNA/mus209 antibody, Pcna antibody, dPCNA antibody, l(2)02448 antibody, l(2)56F antibody, l(2)56Fa antibody, l(2)s1534 antibody, cb16 antibody, fb36g03 antibody, wu:fa28e03 antibody, wu:fb36g03 antibody, pcna-A antibody, proliferating cell nuclear antigen antibody, DNA polymerase III sliding clamp antibody, Proliferating cell nuclear antigen antibody, Proliferating cell nuclear antigen-like antibody, proliferating cell nuclear antigen L homeolog antibody, proliferating cell nuclear antigen 1 antibody, PCNA antibody, Pcna antibody, pcna antibody, MMP_RS08810 antibody, ANI_1_512014 antibody, AOR_1_1500154 antibody, MGC53867 antibody, pcna.L antibody, pcna1 antibody, LOC106420145 antibody, pcn1 antibody

Background

Full Gene Name: proliferating cell nuclear antigen
Synonyms: MGC8367

Gene ID

5111

Pathways

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy