ATM antibody (AA 980-1512)

Catalog No. ABIN302280

The Mouse Monoclonal anti-ATM antibody (Clone 3-00E-008) (ABIN302280) specifically detects ATM in WB, IP and IHC (p). The antibody is reactive with Human samples.

Quick Overview for ATM antibody (AA 980-1512) (ABIN302280)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATM antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3-00E-008

Product Details anti-ATM Antibody

Binding Specificity: AA 980-1512

Specificity: This antibbody detects Ataxia Telangiectasia Mutated (ATM).

Characteristics: Synonyms: Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATDC, TEL1, TELO1

Purification: Protein G chromatography

Immunogen: Recombinant protein expressed in E. coli corresponding to amino acids 980-1512

Isotype: IgG1

Application Details

Application Notes: Immonoprecipitation. Western blot. Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C to -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM

Background: ATM-3E8 recognizes full-length ATM, a 370 kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation. The ATM gene product is a 370 kD nuclear phosphoprotein with C-terminal homology to phosphatidylinositol 3-kinase. ATM is involved in the induction of a DNA damage control pathway. Mutations in the ATM gene cause neuronal degeneration, gonadal abnormalities, and immune deficiencies as part of the hereditary disease ataxia telangiectasia.Synonyms: A-T mutated, ATDC, Ataxia telangiectasia mutated, Serine-protein kinase ATM, TEL1, TELO1

Gene ID: 472

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus