TGFA antibody (Transforming Growth Factor, alpha) (C-Term)

Details for Product anti-TGFA Antibody No. ABIN3026161, Supplier: Log in to see
Antigen
  • TGFA
  • LOC100009482
  • NV15606
  • RATTGFAA
  • TGFAA
  • wa-1
  • wa1
  • TFGA
  • TGF alpha
  • transforming growth factor, alpha
  • transforming growth factor alpha
  • transforming growth factor-alpha
  • tgfa
  • TGFA
  • LOC100009482
  • LOC100121300
  • Tgfa
  • TGF-A
  • LOC100009150
  • LOC100358936
Alternatives
anti-Human TGFA antibody for Western Blotting
Epitope
C-Term
101
76
16
6
5
5
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
341
121
117
92
70
36
6
3
2
2
Host
Mouse
204
138
17
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
This TGFA antibody is un-conjugated
26
13
10
9
8
8
7
7
7
7
7
7
7
7
7
7
7
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
179
174
164
157
145
116
114
64
62
18
14
13
8
4
4
2
2
2
1
1
Options
Supplier
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Supplier Product No.
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Immunogen A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
Clone MF9
Isotype IgG1, kappa
Purification Protein G affinity chromatography
Alternative Name TGF alpha (TGFA Antibody Abstract)
Background TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
Gene ID 7039
Research Area Cancer, Angiogenesis, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissues requires boiling sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.\. Western blot: 1-2 μg/mL, FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)

Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Supplier Images
Immunohistochemistry (IHC) image for anti-Transforming Growth Factor, alpha (TGFA) (C-Term) antibody (ABIN3026161) IHC testing of FFPE human testicular carcinoma with TGF alpha antibody
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