PUMA antibody (C-Term)

Catalog No. ABIN302789

The Rabbit Polyclonal anti-PUMA antibody is suitable to detect PUMA in samples from Human and Mouse. It has been validated for WB, IF and IHC (p).

Quick Overview for PUMA antibody (C-Term) (ABIN302789)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PUMA Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of PUMA-alpha. This sequence is identical between alpha and beta forms of the PUMA proteins.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide from the C-terminal region of Human PUMA

Application Details

Application Notes: Western Blot: 1 - 2 μg/mL. Immunohistochemistry: 10 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C to -70 °C for longer.

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis

Gene ID: 27113

NCBI Accession: NP_001120712

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity