PUMA antibody (C-Term)
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- Target See all PUMA (BBC3) Antibodies
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PUMA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.
- Purification
- Immunoaffinity purified
- Isotype
- IgG
- Top Product
- Discover our top product BBC3 Primary Antibody
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anti-BCL2 Binding Component 3 (BBC3) antibody
BBC3 Reactivity: Human WB, IHC, IF Host: Rabbit Monoclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibodyBBC3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 131-180) antibodyBBC3 Reactivity: Human, Rat, Pig WB, ELISA, FACS, IP, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibodyBBC3 Reactivity: Human WB, FACS, IHC (p), IF Host: Rabbit Polyclonal RB01353 unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibodyBBC3 Reactivity: Human WB, ELISA, IHC, FACS, IF Host: Rabbit Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibodyBBC3 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PE)BBC3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal PE
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PerCP)BBC3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal PerCP
anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody (Biotin)BBC3 Reactivity: Human WB, IHC Host: Rabbit Polyclonal Biotin
anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibodyBBC3 Reactivity: Human, Mouse WB, FACS, IHC (p), IF, EIA Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Approved: ICC, IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles. Store undiluted.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Alternative Name
- PUMA (BBC3 Products)
- Synonyms
- BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody
- Background
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Name/Gene ID: BBC3
Family: Apoptosis
Synonyms: BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA - Gene ID
- 27113
- UniProt
- Q9BXH1
- Pathways
- p53 Signaling, Positive Regulation of Endopeptidase Activity
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