RNF25 antibody (C-Term)
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- Target See all RNF25 Antibodies
- RNF25 (Ring Finger Protein 25 (RNF25))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This RNF25 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Purification
- Immunoaffinity Chromatography.
- Immunogen
- Synthetic peptide corresponding to Amino Acids near the Carboxyl terminus of human RNF25 protein.
- Top Product
- Discover our top product RNF25 Primary Antibody
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- Application Notes
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Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunoprecipitation. Western Blot (1/200-1/2000). ELISA (1/10000-1/40000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.
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- Target
- RNF25 (Ring Finger Protein 25 (RNF25))
- Alternative Name
- RNF25 (RNF25 Products)
- Synonyms
- AO7 antibody, 0610009H16Rik antibody, ring finger protein 25 antibody, RNF25 antibody, Rnf25 antibody
- Background
- RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues. RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait. RNF25 has also been shown to act as a putative E3 ligase, at least in vitro. RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kB by interacting with p65 in vivo upon stimulation with TNF. Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates. Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).Synonyms: E3 ubiquitin-protein ligase RNF25, RING finger protein 25
- Gene ID
- 64320
- NCBI Accession
- NP_071898
- UniProt
- Q96BH1
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