RNF25 antibody (Middle Region)

Catalog No. ABIN2774774

The Rabbit Polyclonal anti-RNF25 antibody is suitable to detect RNF25 in samples from Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow and Rabbit. It has been validated for WB and IHC.

Quick Overview for RNF25 antibody (Middle Region) (ABIN2774774)

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Cow, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RNF25 Antibody

Binding Specificity: Middle Region

Purpose: RNF25 Antibody - middle region

Sequence: CREPLVYDLA SLKAAPEPQQ PMELYQPSAE SLRQQEERKR LYQRQQERGG

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 92%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RNF25. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNF25

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8110874

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RNF25

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Alternative Name: RNF25

Background:

Background Information: RNF25 contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.

Gene Name: Ring finger protein 25

Alternative Symbols: AO7

Protein Name: E3 ubiquitin-protein ligase RNF25

Molecular Weight: 51kDa

Gene ID: 64320

NCBI Accession: NP_071898

UniProt: Q96BH1