RNF25 antibody (C-Term)

Catalog No. ABIN129713

Product Details anti-RNF25 Antibody

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the of human RNF25 protein.

Isotype: IgG

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA, western blot and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 54 kDa in size corresponding to RNF25 protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for RNF25

Target: RNF25 (Ring Finger Protein 25 (RNF25))

Alternative Name: RNF25 (RNF25 Products)

Synonyms: AO7 antibody, 0610009H16Rik antibody, ring finger protein 25 antibody, RNF25 antibody, Rnf25 antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues.  RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait.  RNF25 has also been shown to act as a putative E3 ligase, at least in vitro.  RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kB by interacting with p65 in vivo upon stimulation with TNF.  Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates.  Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).
Synonyms: AO7 antibody, E3 ubiquitin protein ligase RNF25 antibody, EC 6.3.2.- antibody, FLJ13906 antibody, Ring finger protein 25 antibody, RING finger protein AO7 antibody, RNF 25 antibody

Gene ID: 64320, 34878787

UniProt: Q96BH1