HSD17B6 antibody (C-Term)

Catalog No. ABIN3042725

Product Details anti-HSD17B6 Antibody

Target: HSD17B6 (Hydroxysteroid (17-Beta) Dehydrogenase 6 (HSD17B6))

Binding Specificity: AA 300-317, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for 17-beta-hydroxysteroid dehydrogenase type 6(HSD17B6) detection. Tested with WB, IHC-P in Human.

Sequence: SLADYILTRS WPKPAQAV

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for 17-beta-hydroxysteroid dehydrogenase type 6(HSD17B6) detection. Tested with WB, IHC-P in Human.
Gene Name: hydroxysteroid(17-beta) dehydrogenase 6 homolog(mouse)
Protein Name: 17-beta-hydroxysteroid dehydrogenase type 6(17-beta-HSD 6/17-beta-HSD6)

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B6(300-317aa SLADYILTRSWPKPAQAV).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HSD17B6

Target: HSD17B6 (Hydroxysteroid (17-Beta) Dehydrogenase 6 (HSD17B6))

Alternative Name: HSD17B6 (HSD17B6 Products)

Synonyms: HSE antibody, RODH antibody, SDR9C6 antibody, 17betaHSD9 antibody, Hsd17b9 antibody, Rdh8 antibody, hydroxysteroid 17-beta dehydrogenase 6 antibody, hydroxysteroid (17-beta) dehydrogenase 6 antibody, HSD17B6 antibody, Hsd17b6 antibody

Background: Hydroxysteroid 17-beta dehydrogenase 6 is an enzyme that in humans is encoded by the HSD17B6 gene. The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. Baker ME et al point out expression of 17beta-HSDs had an important role in the early evolution of the physiological response to androgens and estrogens. Biswas and Russell concluded that 17beta-HSD6 and RoDH play opposing roles in androgen action, 17beta-HSD6 inactivates 3alpha-adiol by conversion to androsterone and RoDH activates 3alpha-adiol by conversion to dihydrotestosterone. The synthesis of an active steroid hormone by back conversion of an inactive metabolite represents a potentially important mechanism by which the steady state level of a transcriptional effector can be regulated.

Synonyms: 17-beta-HSD 6 antibody|17-beta-HSD6 antibody|17-beta-hydroxysteroid dehydrogenase type 6 antibody|3 hydroxysteroid epimerase antibody|3(alpha>beta) hydroxysteroid epimerase antibody|3(alpha>beta) hydroxysteroid epimerasel antibody|3-alpha->beta-HSE antibody|3-alpha->beta-hydroxysteroid epimerase antibody|H17B6_HUMAN antibody|HSD17B6 antibody|HSE antibody|Hydroxysteroid(17 beta) dehydrogenase 6 antibody|Hydroxysteroid(17 beta) dehydrogenase 6 homolog(mouse) antibody|Hydroxysteroid 17 beta dehydrogenase 6 antibody|NAD+ dependent 3 alpha hydroxysteroid dehydrogenase 3 hydroxysteroid epimerase antibody|NAD+ dependent 3 alpha hydroxysteroid dehydrogenase antibody|Oxidative 3 alpha hydroxysteroid dehydrogenase antibody|Oxidative 3-alpha hydroxysteroid dehydrogenase antibody|Oxidoreductase antibody|Retinol dehydrogenase antibody|RODH antibody|SDR9C6 antibody|Short chain dehydrogenase/reductase family 9C, member 6 antibody

UniProt: O14756

Pathways: Steroid Hormone Biosynthesis