CYP7A1 antibody (AA 301-504)

Catalog No. ABIN3042786

Product Details anti-CYP7A1 Antibody

Target: CYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1))

Binding Specificity: AA 301-504

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP7A1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Cholesterol 7-alpha-monooxygenase(CYP7A1) detection. Tested with WB in Human,Mouse,Rat.

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Cholesterol 7-alpha-monooxygenase(CYP7A1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: cytochrome P450, family 7, subfamily A, polypeptide 1
Protein Name: Cholesterol 7-alpha-monooxygenase

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CYP7A1 recombinant protein (Position: M301-L504). Human CYP7A1 shares 85.2 % and 83.3% amino acid (aa) sequence identity with mouse and rat CYP7A1, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CYP7A1

Target: CYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1))

Alternative Name: CYP7A1 (CYP7A1 Products)

Synonyms: CP7A antibody, CYP7 antibody, CYPVII antibody, CHAP antibody, CYP7S1 antibody, cyp7a1a antibody, zgc:63920 antibody, cytochrome P450 family 7 subfamily A member 1 antibody, cytochrome P450, family 7, subfamily a, polypeptide 1 antibody, cytochrome P450, family 7, subfamily A, polypeptide 1 antibody, cholesterol 7-alpha-hydroxylase antibody, CYP7A1 antibody, Cyp7a1 antibody, cyp7a1 antibody

Background: CYP7A1 (Cytochrome P450 Subfamily VIIA Polypeptide 1), also called CYP7 or CHOLESTEROL 7-ALPHA -HYDROXYLASE, is an enzyme that in humans is encoded by the CYP7A1 gene. Using both mouse-human somatic cell hybrids and in situ chromosomal hybridization, Cohen et al. (1992) mapped the CYP7 gene to 8q11-q12. By transfection of reporter constructs, mutation analysis, and DNase footprinting, Molowa et al. (1992) identified areas of the CYP7A1 promoter region that showed hepatocyte-specific activation. They found HNF3 to be an activator of CYP7A1 activity. Agellon et al. (2002) found that wildtype mice and mice transgenic for human CYP7A1 respond differently to cholesterol feeding. Cholesterol feeding stimulated Cyp7a1 mRNA abundance and enzymatic activity in wildtype mice, but repressed human CYP7A1 mRNA and activity in transgenic mice.

Synonyms: Cholesterol 7 alpha hydroxylase antibody|Cholesterol 7 alpha monooxygenase antibody| cholesterol 7 alpha-monooxygenase antibody|Cholesterol 7-alpha-hydroxylase antibody| Cholesterol 7-alpha-monooxygenase antibody|CP7A antibody|CP7A1_HUMAN antibody|CYP 7 antibody|CYP7 antibody|CYP7A1 antibody|CYPVII antibody|Cytochrome P450 7A1 antibody| cytochrome P450, family 7, subfamily A, polypeptide 1 antibody|Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), antibody|cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), antibody|Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1 antibody|MGC126826 antibody|MGC138389 antibody

Gene ID: 1581

UniProt: P22680

Pathways: Steroid Hormone Biosynthesis, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha