NOX5 antibody (C-Term)
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- Target See all NOX5 Antibodies
- NOX5 (NADPH Oxidase 5 (NOX5))
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Binding Specificity
- AA 747-765, C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NOX5 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for NADPH oxidase 5(NOX5) detection. Tested with WB, IHC-P, ICC in Human.
- Sequence
- KVLKGHCEKF GFRFFQENF
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for NADPH oxidase 5(NOX5) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: NADPH oxidase, EF-hand calcium binding domain 5
Protein Name: NADPH oxidase 5 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the C-terminus of human NOX5(747-765aa KVLKGHCEKFGFRFFQENF).
- Isotype
- IgG
- Top Product
- Discover our top product NOX5 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasion in HeLa Cells." in: PLoS ONE, Vol. 12, Issue 1, pp. e0170327, (2017) (PubMed).
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CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasion in HeLa Cells." in: PLoS ONE, Vol. 12, Issue 1, pp. e0170327, (2017) (PubMed).
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- Target
- NOX5 (NADPH Oxidase 5 (NOX5))
- Alternative Name
- NOX5 (NOX5 Products)
- Synonyms
- NADPH oxidase 5 antibody, NOX5 antibody
- Background
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NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al.(2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al.(2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells(HC), but not normal B cells.
Synonyms: MGC149776 antibody|MGC149777 antibody|NADPH oxidase 5 antibody|NADPH oxidase EFhand calcium binding domain 5 antibody|NOX5 antibody|NOX5_HUMAN antibody - Pathways
- Proton Transport
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