SOX1 antibody (Middle Region)

Catalog No. ABIN3043439

Product Details anti-SOX1 Antibody

Target: SOX1 (SRY (Sex Determining Region Y)-Box 1 (SOX1))

Binding Specificity: AA 215-248, Middle Region

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Transcription factor SOX-1(SOX1) detection. Tested with WB in Human,Mouse,Rat.

Sequence: QHPGAGGAHP HAHPAHPHPH HPHAHPHNPQ PMHR

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Transcription factor SOX-1(SOX1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: SRY (sex determining region Y)-box 1
Protein Name: Transcription factor SOX-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of Human SOX1(215-248aa QHPGAGGAHPHAHPAHPHPHHPHAHPHNPQPMHR), identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human , The detection limit for SOX1 is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-SOX1 antibody (ABIN3043439)

Yi, Xie, Liu, Wang, Xu, Liu, Li, Zhong, Peng: "Derivation and Identification of Motor Neurons from Human Urine-Derived Induced Pluripotent Stem Cells." in: Stem cells international, Vol. 2018, pp. 3628578, (2018) (PubMed).

Target Details for SOX1

Target: SOX1 (SRY (Sex Determining Region Y)-Box 1 (SOX1))

Alternative Name: SOX1 (SOX1 Products)

Synonyms: BB176347 antibody, Sox-1 antibody, XlSox1 antibody, xSox1 antibody, wu:fi38c08 antibody, wu:fj38h03 antibody, zgc:92865 antibody, SRY-box 1 antibody, SRY (sex determining region Y)-box 1 antibody, SRY-box 1 S homeolog antibody, SRY (sex determining region Y)-box 1b antibody, SRY box 1 antibody, SRY (sex determining region Y)-box 1a antibody, SOX1 antibody, Sox1 antibody, sox1.S antibody, sox1b antibody, sox1a antibody

Background: SOX1 (for Sex determining region Y-box 1), located in 13q34, is a transcription factor in the Sox protein family. It means that this intronless gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development. SOX1 expression is restricted to neuroectodermin the tetrapod embryo. It is expressed particularly in the ventral striatum.

Synonyms: Sex determining region Y box 1 antibody|sox1 antibody|SOX1_HUMAN antibody|SRY antibody|SRY box containing gene 1 antibody|SRY related HMG box gene 1 antibody|Transcription factor Sox-1 antibody

Gene ID: 6656

UniProt: O00570