P2RX2 antibody (AA 139-471)

Catalog No. ABIN3043588

Product Details anti-P2RX2 Antibody

Target: P2RX2 (Purinergic Receptor P2X, Ligand Gated Ion Channel 2 (P2RX2))

Binding Specificity: AA 139-471

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for P2X purinoceptor 2(P2RX2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for P2X purinoceptor 2(P2RX2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: purinergic receptor P2X, ligand gated ion channel, 2
Protein Name: P2X purinoceptor 2

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human P2X2 recombinant protein (Position: D139-L471). Human P2X2 shares 84% amino acid (aa) sequence identity with both mouse and rat P2X2.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for P2X2 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-P2RX2 antibody (ABIN3043588)

Meng, Zheng, Yan, Li, Fang, Li: "P2X2 and P2X5 Receptors Mediate Bladder Hyperesthesia in ICC in Female Overactive Bladder." in: Cell biochemistry and biophysics, Vol. 72, Issue 2, pp. 375-83, (2016) (PubMed).

Lin, Li, Den, Xu, Liu, Gao, Liu, Zhang, Li, Liang: "VEGF and its receptor-2 involved in neuropathic pain transmission mediated by P2X?(/)? receptor of primary sensory neurons." in: Brain research bulletin, Vol. 83, Issue 5, pp. 284-91, (2010) (PubMed).

Target Details for P2RX2

Target: P2RX2 (Purinergic Receptor P2X, Ligand Gated Ion Channel 2 (P2RX2))

Alternative Name: P2RX2 (P2RX2 Products)

Synonyms: DFNA41 antibody, P2X2 antibody, P2X2a antibody, P2x2 antibody, p2xr2 antibody, P2RX2 antibody, purinergic receptor P2X 2 antibody, purinergic receptor P2X, ligand-gated ion channel, 2 antibody, P2RX2 antibody, P2rx2 antibody, p2rx2 antibody

Background: The P2RX2 gene encodes the P2X2 receptor, which assembles as a trimer to form a ligand-gated ion channel gated by extracellular ATP. P2X2 receptors mediate a variety of cellular responses, including excitatory postsynaptic responses in sensory neurons. The product of this gene belongs to the family of purinoceptors for ATP. P2RX2 is mapped to 12q24.33. It has been found that ATP-activated P2RX2 influenced OHC electromotility, a stimulus-induced change in hair cell length that functions as an amplifier to determine hearing sensitivity and frequency selectivity. What's more, P2RX2 channels were necessary for development of the temporary threshold shift.

Synonyms: ATP receptor antibody|MGC129601 antibody|P2RX 2 antibody|P2RX2 antibody|P2RX2_HUMAN antibody|P2X purinoceptor 2 antibody|P2X Receptor subunit 2 antibody|P2X2 antibody|P2X2a antibody|Purinergic receptor antibody|Purinergic receptor P2X ligand gated ion channel 2 antibody|Purinergic receptor P2X2 isoform A antibody|Purinergic receptor P2X2 isoform B antibody|Purinergic receptor P2X2 isoform C antibody|Purinergic receptor P2X2 isoform D antibody|Purinergic receptor P2X2 isoform H antibody|Purinergic receptor P2X2 isoform I antibody

Gene ID: 22953

Pathways: Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity