ABCB4 antibody (AA 601-720)

Catalog No. ABIN3043734

Product Details anti-ABCB4 Antibody

Target: ABCB4 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 4 (ABCB4))

Binding Specificity: AA 601-720

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCB4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Phosphatidylcholine translocator ABCB4(ABCB4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Phosphatidylcholine translocator ABCB4(ABCB4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: ATP-binding cassette, sub-family B (MDR/TAP), member 4
Protein Name: Phosphatidylcholine translocator ABCB4

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ABCB4 recombinant protein (Position: A601-A720). Human ABCB4 shares 79% amino acid (aa) sequence identity with both mouse and rat ABCB4.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for ABCB4 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for ABCB4

Target: ABCB4 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 4 (ABCB4))

Alternative Name: ABCB4 (ABCB4 Products)

Synonyms: ABC21 antibody, GBD1 antibody, ICP3 antibody, MDR2 antibody, MDR2/3 antibody, MDR3 antibody, PFIC-3 antibody, PGY3 antibody, zgc:172149 antibody, Mdr2 antibody, Pgy-2 antibody, Pgy2 antibody, mdr-2 antibody, Pgy3 antibody, ABCB4a antibody, DEFB1 antibody, PGP2 antibody, PGY2 antibody, PGP3 antibody, ABCB4 antibody, RUNDC3B antibody, ATP binding cassette subfamily B member 4 antibody, ATP-binding cassette, sub-family B (MDR/TAP), member 4 antibody, beta-defensin 1-like antibody, ATP-binding cassette, sub-family B (MDR/TAP), member 1B antibody, RUN domain-containing protein 3B antibody, ABCB4 antibody, abcb4 antibody, Abcb4 antibody, LOC100861170 antibody, Abcb1b antibody, LOC101122517 antibody

Background: Adenosine triphosphate-binding cassette subfamily B, member 4 (ABCB4), also called MDR3, is a protein that in humans is encoded by the ABCB4 gene. The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. The ABCB4 gene contains 28 exons and 27 of these contain coding sequences for the two homologous halves of the protein that correlate with functional domains. ABCB4 gene mutations represent a genetic factor involved in this peculiar form of cholesterol gallstone disease in adults.

Synonyms: ABC 21 antibody|ABC B4 antibody|ABC21 antibody|ABCB 4 antibody|Abcb4 antibody|ABCB4 protein antibody|ATP binding cassette sub family B MDR/TAP member 4 antibody|ATP binding cassette sub family B member 4 antibody|ATP-binding cassette sub-family B member 4 antibody|MDR 3 antibody|MDR2 antibody|MDR2/3 antibody|MDR3 antibody|MDR3 P glycoprotein antibody|MDR3 P gp antibody|MDR3_HUMAN antibody|Multidrug resistance protein 3 antibody|Multiple drug resistance 3 antibody|P glycoprotein 3 antibody|P-glycoprotein 3 antibody|PFIC 3 antibody|PFIC3 antibody|PGY 3 antibody|PGY3 antibody

Gene ID: 5244

UniProt: P21439

Pathways: Regulation of Lipid Metabolism by PPARalpha