Hsc70 antibody (AA 520-614)
-
- Target See all Hsc70 (HSPA8) Antibodies
- Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
-
Binding Specificity
- AA 520-614
-
Reactivity
- Human, Rat, Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This Hsc70 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Heat shock cognate 71 kDa protein(HSPA8) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
-
Rabbit IgG polyclonal antibody for Heat shock cognate 71 kDa protein(HSPA8) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: heat shock 70 kDa protein 8
Protein Name: Heat shock cognate 71 kDa protein - Purification
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human Hsc70 recombinant protein (Position: Q520-A614). Human Hsc70 shares 98.9% amino acid (aa) sequence identity with both mouse and rat Hsc70.
- Isotype
- IgG
- Top Product
- Discover our top product HSPA8 Primary Antibody
-
-
- Application Notes
-
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
-
- Target
- Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
- Alternative Name
- HSPA8 (HSPA8 Products)
- Synonyms
- hsc54 antibody, hsc70 antibody, hsc71 antibody, hsp71 antibody, hsp73 antibody, hspa10 antibody, lap1 antibody, nip71 antibody, HSC54 antibody, HSC70 antibody, HSC71 antibody, HSP71 antibody, HSP73 antibody, HSPA10 antibody, LAP1 antibody, NIP71 antibody, Hsc70 antibody, 2410008N15Rik antibody, Hsc71 antibody, Hsc73 antibody, Hsp73 antibody, Hspa10 antibody, wu:fb01g06 antibody, wu:fi48b06 antibody, heat shock protein family A (Hsp70) member 8 L homeolog antibody, heat shock protein family A (Hsp70) member 8 antibody, heat shock 70kDa protein 8 antibody, heat shock protein 8 antibody, hspa8.L antibody, HSPA8 antibody, Hspa8 antibody, hspa8 antibody
- Background
-
HSPA8 (heat shock 70 kDa protein 8) also known as HSC70, HSC71, HSP73, HSPA10, FORMERLY, LAP1 or LPS-ASSOCIATED PROTEIN 1, is a heat shock protein that in humans is encoded by the HSPA8 gene. The HSPA8 gene contains 9 exons and spans 5 kb. The deduced HSPA8 protein has 646 amino acids and a predicted molecular mass of 70,899 Da. And the HSPA8 gene is mapped on 11q24.1. HSPA8 plays an important role in cells by transiently associating with nascent polypeptides to facilitate correct folding. HSP73 also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Rapid decay involves AU-rich binding protein AUF1, which complexes with heat-shock proteins HSC70 and HSP70, translation initiation factor EIF4G, and poly (A)-binding protein. In the absence of Il3, Hsc70 formed a complex with Hsp40 and Hip, and this complex, in association with Eif4g and Pabp, formed a high-stability complex with Bim mRNA that protected it from ribonucleases.
Synonyms: 2410008N15Rik antibody|Constitutive heat shock protein 70 antibody|Heat shock 70 kDa protein 8 antibody|Heat shock 70kD protein 10 antibody|Heat shock 70kD protein 8 antibody| Heat shock 70 kDa protein 8 antibody|Heat shock cognate 71 kDa protein antibody| Heat shock cognate 71 kDa protein. antibody|Heat shock cognate protein 54 antibody|Heat shock cognate protein 71 kDa antibody|Heat shock cognate protein 71- kDa antibody|Heat shock protein 8 antibody|Heat shock protein A8 antibody|Heat-shock70-KD protein 10, formerly antibody|HSC54 antibody|HSC71 antibody|Hsc73 antibody|HSP71 antibody|HSP73 antibody|HSP7C_HUMAN antibody| HSPA10 antibody|HSPA8 antibody|LAP1 antibody|Lipopolysaccharide associated protein 1 antibody|LPS associated protein 1 antibody|LPS associated protein antibody|MGC102007 antibody|MGC106514 antibody|MGC114311 antibody|MGC118485 antibody|MGC131511 antibody| MGC29929 antibody|N-myristoyltransferase inhibitor protein 71 antibody|NIP71 antibody - Gene ID
- 3312
- UniProt
- P11142
-