IDH2 antibody (C-Term)

Catalog No. ABIN3043856

Product Details anti-IDH2 Antibody

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Binding Specificity: AA 413-447, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Isocitrate dehydrogenase [NADP], mitochondrial(IDH2) detection. Tested with WB, IHC-P in Human, Mouse,Rat.

Sequence: KDLAGCIHGL SNVKLNEHFL NTTDFLDTIK SNLDR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Isocitrate dehydrogenase [NADP], mitochondrial(IDH2) detection. Tested with WB, IHC-P in Human, Mouse,Rat.
Gene Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Protein Name: Isocitrate dehydrogenase [NADP], mitochondrial

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IDH2 (413-447aa KDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDR), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for IDH2

Target: IDH2 (Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial (IDH2))

Alternative Name: IDH2 (IDH2 Products)

Synonyms: F6P23.14 antibody, F6P23_14 antibody, IDH-II antibody, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 2 antibody, isocitrate dehydrogenase II antibody, isocitrate dehydrogenase subunit 2 antibody, D2HGA2 antibody, ICD-M antibody, IDH antibody, IDHM antibody, IDP antibody, IDPM antibody, mNADP-IDH antibody, E430004F23 antibody, IDPm antibody, Idh-2 antibody, wu:fb33c06 antibody, wu:fk31e05 antibody, wu:fq43b01 antibody, zgc:55485 antibody, idh2 antibody, IDH2 antibody, isocitrate dehydrogenase subunit 2 antibody, isocitrate dehydrogenase (NADP(+)) 2, mitochondrial antibody, isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody, isocitrate dehydrogenase 2 (NADP+), mitochondrial S homeolog antibody, isocitrate dehydrogenase [NADP], mitochondrial antibody, IDH2 antibody, Idh2 antibody, idh2.S antibody, idh2 antibody, LOC100441867 antibody

Background: Isocitrate dehydrogenase [NADP], mitochondrial is an enzyme that in humans is encoded by the IDH2 gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.

Synonyms: D2HGA2 antibody|ICD-M antibody|IDH antibody|IDH2 antibody|IDHM antibody|IDHP_HUMAN antibody|IDP antibody|IDPM antibody|Isocitrate dehydrogenase [NADP], mitochondrial antibody|Isocitrate dehydrogenase 2 (NADP+), mitochondrial antibody|mNADP-IDH antibody|NADP(+)-specific ICDH antibody|Oxalosuccinate decarboxylase antibody

Gene ID: 3418

UniProt: P48735

Pathways: Warburg Effect