TR4 (NR2C2)
(Nuclear Receptor Subfamily 2, Group C, Member 2 (NR2C2))
Reactivity
Human
Host
Sheep
Clonality
Polyclonal
Conjugate
This TR4 antibody is un-conjugated
Application
Western Blotting (WB), Immunoprecipitation (IP)
Cross-Reactivity
Human
Characteristics
Mark3, Sheep anti-Cdc25c associated protein kinase-1, human,C-TAK1 (Cdc25C associated protein kinase) is a ubiquitously expressed protein kinase found in human tissues and cell lines and can phosphorylate Cdc25C on serine 216 in vitro. C-TAK1 is distinct from the DNA damage checkpoint kinase Chk1 and may regulate interactions between Cdc25C and 14-3-3 in vivo by phosphorylating Cdc25C at serine 216.
Purification
Ammonium Sulfate Precipitation
Immunogen
Recombinant, full-length His-tagged human C-TAK1 produced in E. Coli.
Antibody detects c-tak1 by Western blot analysis at 1 to 5 μg/mL. Detects c-tak1 in RKO and HCT116 cell lysates. Optimal concentration should be evaluated by serial dilutions.
Restrictions
For Research Use only
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Target
TR4 (NR2C2)
(Nuclear Receptor Subfamily 2, Group C, Member 2 (NR2C2))