RIT1 antibody

Catalog No. ABIN306805

Product Details anti-RIT1 Antibody

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIT1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Characteristics: Rit and its neuron-specific homologue, Rin, define a recently discovered subfamily of Ras-related GTPases. Rit and Rin are membrane-associated in spite of the fact that they lack a CAAX box or similar C-terminal lipidation motif. Rit and Rin display 64 % amino acid sequence identity and share a unique nine amino acid effector domain (DPTIEDAYK) that is 100 % conserved between the murine and human proteins. Although the effector domain sequences of Rit and Rin are very similar to that of Ras, Rit and Rin have been shown to interact with the known Ras-binding proteins RalGDS, Rlf and AF-6, but not the Raf kinases, RIN1 or the p110 subunit of PI3 kinase. For this reason, it has been suggested that Rit and Rin may play important roles in the regulation of signaling pathways distinct from those controlled by Ras.

Purification: Ammonium Sulfate Precipitation

Immunogen: His-tagged recombinant human Rit protein

Isotype: IgG

Application Details

Application Notes: Antibody detects recombinant Rit protein expressed in HEK-293 cells by Western blot analysis. Shows minimal cross-reactivity with Rin protein and other Ras proteins. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Handling

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Details for RIT1

Target: RIT1 (Ras-Like Without CAAX 1 (RIT1))

Alternative Name: Rit (RIT1 Products)

Synonyms: NS8 antibody, RIBB antibody, RIT antibody, ROC1 antibody, RIT1 antibody, rit1 antibody, MGC79097 antibody, RGD1559874 antibody, si:ch211-93a2.3 antibody, Rit antibody, Ras like without CAAX 1 antibody, Ras-like without CAAX 1 L homeolog antibody, Ras-like without CAAX 1 antibody, RIT1 antibody, rit1.L antibody, Rit1 antibody, rit1 antibody

UniProt: Q92963

Pathways: Neurotrophin Signaling Pathway