Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Cross-Reactivity
Chicken, Cow, Human, Monkey, Mouse, Rat
Characteristics
Synaptosome-Associated Protein of 25 kDa,SNAP25 (Synaptosome-Associated Protein of 25 kDa) is a membrane bound, pre-synaptic nerve terminal protein that plays an essential role in synaptic vesicle fusion and exocytosis. It is involved in the molecular regulation of neurotransmitter release. It is thought to play an important role in the synaptic function of specific neuronal systems. SNAP25 associates with proteins involved in vesicle docking and membrane fusion. SNAP25 exists as two alternatively spliced isoforms, SNAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells. These two isoforms differ by 9 amino acids in the central portion of the protein.
Purification
Protein A/G Chromatography
Immunogen
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with purified rat SNAP25 and mouse NS1 cells.
Antibody can be used for Western blotting (1-5 μg/mL) and immunohistochemistry on paraformaldehyde fixed CNS tissues (1-5 μg/mL). Does not stain reduced proteins. Optimal concentration should be evaluated by serial dilutions.
Restrictions
For Research Use only
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles