Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Cross-Reactivity
Dog, Human, Mouse, Rat
Characteristics
Calreticulin, a Ca2+ chaperone, is an abundant highly conserved calcium binding protein found in the lumen of the endoplasmic reticulum of eukaryotic cells. This lumen protein can be used to measure the intactness of membrane preparations. It is used as a marker for subcellular fractionation of cells.
Purification
Ammonium Sulfate Precipitation
Immunogen
Sheep were immunized with a recombinant protein derived from the carboxy terminus of the calreticulin protein fused to KLH.
Antibody can be used for Western blotting (1:1000-1:5000 dilution) and immunofluorescence (1:50 dilution). Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjugated to OVA, GST, His tags and other.
Restrictions
For Research Use only
Buffer
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles