Nitrotyrosine antibody

Antigen: Nitrotyrosine

Clonality: Monoclonal (6B2)

Host: Mouse

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN308216

Additional Information

Alternative name: Nitro Tyrosine

Immunogen: Protein-bound nitrotyrosine

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: 6B2

Description: Tyrosine nitration is a posttranslational modification generated by a nitrogen free radical (peroxinitrite ONOO-). This reactive nitrogen specie reacts with macromolecules of the organism (amino acid, protein…) and leads to functional alterations which can either participate to adaptation or lead to cell death during cell-stress conditions

Specificity: Antibody specific of free or protein incorporated nitro tyrosine as human serum nitro albumin, nitro haemoglobin, nitro insulin. Evaluation of specificity by competition ELISA with various analogues of Nitrotyrosine (concentration 100μg/mL): Nitrotyrosine: 95 % of inhibition; Tyrosine: 0 % of inhibition; Nitrotryptophan: 4 % of inhibition; Aminotyrosine: 0 % of inhibition; Phosphotyrosine: 2 % of inhibition; Chlorotyrosine: 3 % of inhibition; (Tested by ELISA)

Comments:

For research use only.

Application Details

Application Notes: Recommended concentrations: indirect ELISA: 2 to 100ng/ml; IP: 1 μg/ml; western blot: 0.1 μg/ml. Optimal dilutions/concentrations should be determined by the end user.

Concentration: 1 mg/ml

Purity: Purified IgG by Affinity chromatography on Protein A

Buffer: Phosphate Buffered Saline 10 mM – NaCl 0,15 M – pH 7,4. Thiemrosol 0,01% is used as preservative.

Storage: Store at -20 C if preferred. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Research Area: Metabolism

Restrictions: For Research Use only

Images

Purity control (SDS PAGE): Polyacrylamide gel 13,5 % under denaturated conditions. Staining: Coomassie Blue. Lane 1: Molecular Weight markers. Lane 2: IgG Isotype Control. Lane 3: ABIN308216 (1ug).