Ribophorin 1 (RPN1) (N-Term) antibody

Details for Product No. ABIN310034
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Antigen
Synonyms OST1, RBPH1, AU018702, D6Wsu137e, Rpn-1, RIBI, wu:fa12h07, wu:fc68b07, wu:fc88a12, ost1, xrpn1
Epitope
N-Term
(13), (7), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Pig (Porcine), Dog (Canine), Human, Chicken
(55), (6), (5), (5), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(34), (23)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (3), (2), (2), (2), (2)
Application
Western Blotting (WB)
(56), (27), (19), (15), (11), (8), (7), (6), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN310034
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human RPN1
Sequence TSRATSFLLA LEPELEARLA HLGVQVKGED EEENNLEVRE TKIKGKSGRF
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Pig : 100 %, Rabbit : 100 %, Mouse : 90 %, Rat : 90 %, Chicken : 70 %  
Characteristics This is a rabbit polyclonal antibody against RPN1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name RPN1
Background RPN1 is a type I integral membrane protein found only in the rough endoplasmic reticulum. It is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. RPN1 forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.
Molecular Weight 69 kDa
Gene ID 6184
NCBI Accession NP_002941, NM_002950
UniProt P04843
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 607 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Ribophorin 1 (RPN1) (N-Term) antibody WB Suggested Anti-RPN1 Antibody Titration: 0.2-1 ug/ml
Positive Control: MCF7 cell lysate
anti-Ribophorin 1 (RPN1) (N-Term) antibody (2) WB Suggested Anti-RPN1 Antibody Titration: 1 µg/mL Positive Control: HeLa and CHO-K1 cell lines, rouch canine microsomes
Product cited in: Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

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