Actinin, alpha 2 (ACTN2) (C-Term) antibody

Details for Product No. ABIN310156
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Antigen
Synonyms MGC89234, ACTN2, 1110008F24Rik, CMD1AA, wu:fd44c03, zgc:123223
Epitope
C-Term
(16), (8), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Xenopus laevis, Cow (Bovine), Zebrafish (Danio rerio), Human, Chicken
(30), (24), (11), (5), (4), (4), (3), (2), (2), (2), (2)
Host
Rabbit
(40), (11)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(44), (17), (14), (11), (9), (8), (5), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN310156
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Immunogen Synthetic peptide directed towards the C terminal of human ACTN2
Sequence VIASFRILAS DKPYILAEEL RRELPPDQAQ YCIKRMPAYS GPGSVPGALD
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, African clawed frog : 90 %, Chicken : 90 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against ACTN2. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name ACTN2
Background The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Transcript variants resulting from the use of multiple poly_A sites have been observed.
Molecular Weight 98 kDa
Gene ID 88
NCBI Accession NP_001094, NM_001103
UniProt P35609
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 894 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Actinin, alpha 2 (ACTN2) (C-Term) antibody anti-Actinin, alpha 2 (ACTN2) (C-Term) antibody
anti-Actinin, alpha 2 (ACTN2) (C-Term) antibody (2) WB Suggested Anti-ACTN2
Antibody Titration: 1.25 µg/mL
Positive Control: Human heart
Background publications Eldstrom, Choi, Steele et al.: "SAP97 increases Kv1.5 currents through an indirect N-terminal mechanism." in: FEBS letters, Vol. 547, Issue 1-3, pp. 205-11, 2003 (PubMed).

Validation Images
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