MAFK (N-Term) antibody

Details for Product No. ABIN310919
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Antigen
Synonyms AW061068, NF-E2, Nfe2u, NFE2U, P18
Epitope
N-Term
(9), (3), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine), Human, Chicken
(18), (2)
Host
Rabbit
(14), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(19), (11), (7), (2), (2)
Pubmed 1 reference available
Catalog no. ABIN310919
Quantity 100 µg
Price
229.00 $   Plus shipping costs $45.00
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  • +1 404 474 4654
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Immunogen Synthetic peptide directed towards the N terminal of human MAFK
Sequence KEAGENAPVLSDDELVSMSVRELNQHLRGLTKEEVTRLKQ RRRTLKNRGY
Predicted Reactivity Human : 100 %, Mouse : 100 %, Rat : 100 %, Chicken : 90 %, Bovine : 80 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against MAFK. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Background The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2. NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, PBGD and FECH. NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG, or MafK) and p45. Both subunits are members of the activator protein-1 superfamily of bZIP proteins. Maf homodimers suppress transcription at NFE2 sites.The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD, MIM 176000) and ferrochelatase (FECH, MIM 177000). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.
Molecular Weight 17 kDa
Gene ID 7975
NCBI Accession NM_002360, NP_002351
UniProt O60675
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 156 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-MAFK (N-Term) antibody WB Suggested Anti-MAFK Antibody Titration: 2.5ug/ml
Positive Control: Jurkat cell lysate
Background publications Hillier, Fulton, Fulton et al.: "The DNA sequence of human chromosome 7." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003 (PubMed).

Hosts (14), (5)
Reactivities (18), (2)
Applications (19), (11), (7), (2), (2)
Conjugates (1), (1), (1), (1), (1), (1)
Epitopes (9), (3), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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