MAFK antibody

Catalog No. ABIN7469481

Product Details anti-MAFK Antibody

Target: MAFK (Transcription Factor MafK (MAFK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAFK antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)

Cross-Reactivity: Human, Mouse

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length human MAFK Recombinant protein.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MAFK

Target: MAFK (Transcription Factor MafK (MAFK))

Alternative Name: MAFK (MAFK Products)

Synonyms: NFE2U antibody, P18 antibody, AW061068 antibody, NF-E2 antibody, Nfe2u antibody, MAF bZIP transcription factor K antibody, v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian) antibody, v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog K antibody, Mafk antibody, MAFK antibody, mafk antibody

Background:

Synonyms: MAF bZIP transcription factor K , NFE2U , P18

Background: The developmentally regulated expression of the globin genes depends on upstream regulatory elements termed locus control regions (LCRs). LCRs are associated with powerful enhancer activity that is mediated by the transcription factor NFE2 (nuclear factor erythroid-2). NFE2 recognition sites are also present in the gene promoters of 2 heme biosynthetic enzymes, porphobilinogen deaminase (PBGD, MIM 609806) and ferrochelatase (FECH, MIM 612386). NFE2 DNA-binding activity consists of a heterodimer containing an 18-kD Maf protein (MafF, MafG (MIM 602020), or MafK) and p45 (MIM 601490). Both subunits are members of the activator protein-1 superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160). Maf homodimers suppress transcription at NFE2 sites.[supplied by OMIM]

Molecular Weight: 18 kDa

Gene ID: 7975

UniProt: O60675