Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (Middle Region) antibody

Details for Product No. ABIN311017
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Antigen
Synonyms MDH, MDH-1, Mdh, Mdh-1, Mdh2, MdhD, cMdh, sMdh, MDH-s, MDHA, MGC:1375, MOR2, B230377B03Rik, D17921, Mor-2, Mor2, MDL1, Mdhl, mdh-s, mdha, mor2
Epitope
Middle Region
(4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Arabidopsis, Mouse (Murine), Rat (Rattus), Pig (Porcine), Cow (Bovine), Human, Chicken
(50), (26), (19), (18), (13), (12), (12), (1)
Host
Rabbit
(38), (11), (11), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(56), (39), (16), (14), (12), (10), (10), (9), (9), (9), (4), (3), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN311017
229.00 $
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Immunogen Synthetic peptide directed towards the middle region of human MDH1
Sequence NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV
Predicted Reactivity Guinea pig : 100 %, Human : 100 %, Pig : 100 %, Dog : 90 %, Zebrafish : 90 %
Characteristics This is a rabbit polyclonal antibody against MDH1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name MDH1
Background Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
Molecular Weight 36 kDa
Gene ID 4190
NCBI Accession NM_005917, NP_005908
UniProt P40925
Research Area Cancer, Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 334 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Malate Dehydrogenase 1, NAD (Soluble) (MDH1) (Middle Region) antibody WB Suggested Anti-MDH1 Antibody Titration: 2.5ug/ml
Positive Control: Jurkat cell lysate
Background publications Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Validation Images
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