ELISA (detection antibody): The CLB/TF-1 antibody has been used successfully to detectsoluble CD142 in ELISA assays, when using clone CLB/TF-5 (product ABIN155195) as a coatingantibody. Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
TRIS buffered saline containing 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
CD142 expression can be induced on monocytes, macrophages and endothelial cells by various stimuli including interleukin 1, tumour necrosis factor and endotoxin. CD142 initiates the blood clotting cascade by binding coagulation factor VIIa, which activates factor IX or factor X by specific limited proteolysis. CD142 also plays an important role in inflammation, angiogenesis, and the pathophysiology of atherosclerosis and cancer.Synonyms: Coagulation factor III, F3, TF, Thromboplastin