TNF alpha antibody

Catalog No. ABIN317259

The Mouse Monoclonal anti-TNF alpha antibody (Clone 195) (ABIN317259) specifically detects TNF alpha in WB, Func and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for TNF alpha antibody (ABIN317259)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 195

Product Details anti-TNF alpha Antibody

Characteristics: Synonyms: TNF, TNF-a, TNFA, TNFSF2, Cachectin, Tumor necrosis factor ligand superfamily member 2

Purification: Purified

Immunogen: Recombinant human tumour necrosis factor alpha produced in Escherichia coli.

Isotype: IgG3

Application Details

Application Notes: Immunohistochemistry on frozen sections. Functional Assays. Immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor (TNF-Alpha)

Background: TNF alpha is a secreted cytokine that induces cell death in a number of tumour cell lines. This antibody does not react with human tumour necrosis factor beta (TNF beta/lymphotoxin). 50 ng of antibody is required to neutralise 1 unit of TNF alpha.Synonyms: Cachectin, TNF, TNF-a, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome