MAP4K3 antibody

Catalog No. ABIN317719

Product Details anti-MAP4K3 Antibody

Target: MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: GLK antibody detects endogenous levels of GLK protein.(region surrounding Gly4)

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/20000approx. 1/40000. Western Blot: 1/500approx. 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for MAP4K3

Target: MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))

Alternative Name: MAP4K3 (MAP4K3 Products)

Synonyms: GLK antibody, MAPKKKK3 antibody, MEKKK 3 antibody, MEKKK3 antibody, RAB8IPL1 antibody, 4833416M01Rik antibody, 4833416M07Rik antibody, 9530052P13Rik antibody, Glk antibody, mitogen-activated protein kinase kinase kinase kinase 3 antibody, MAP4K3 antibody, Map4k3 antibody

Background: Several mammalian kinases have been identified which exhibit sequence similarity to the Saccharomyces cerevisiae serine/threonine kinase STE20. STE20 is involved in relaying signals from G-protein coupled receptors to cytosolic MAP kinase cascades, and it lies upstream of a MAP kinase kinase kinase. Mammalian STE20-like kinases include GLK, KHS, NIK, YSK1, HPK1, Krs-1, Krs-2 and human GC kinase. GLK (for GC-like kinase) is an 885 amino acid protein that shares a high degree of homology with GC kinase and HPK1. Like many of the STE20-like kinases, GLK specifically activates the JNK pathway. Epistasis studies with dominant negative mutants of MEKK1 suggest that GLK functions upstream of MEKK1 in the JNK signaling pathway.Synonyms: Germinal center kinase-related protein kinase, MAPK, MAPK/ERK kinase kinase kinase 3, MEKKK 3, Mitogen-activated protein kinase kinase kinase kinase 3, RAB8IPL1

Molecular Weight: approx. 101 kDa

Gene ID: 8491

NCBI Accession: NP_003609

UniProt: Q8IVH8

Pathways: MAPK Signaling