CHEK1 antibody (pSer345)

Catalog No. ABIN317989

This Rabbit Polyclonal antibody specifically detects CHEK1 in IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CHEK1 antibody (pSer345) (ABIN317989)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CHEK1 Antibody

Binding Specificity: pSer345

Specificity: This antibody detects endogenous levels of Chk1 pSer345 protein.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: ELISA: 1/10000approx. 1/20000. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % Sodium Azide, approx. pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHK1

Background: Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee 1 in vitro, providing evidence that the hyperphosphorylated form of Wee 1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. Chk1 is phosphorylated on Serine 345 (S345) in response to UV, IR and hydroxyurea (HU). Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development and tumor suppression.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1

Molecular Weight: approx. 56 kDa

Gene ID: 1111

NCBI Accession: NP_001107593

UniProt: O14757

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair