DLL4 antibody (Internal Region)

Catalog No. ABIN318838

Product Details anti-DLL4 Antibody

Target: DLL4 (delta-Like 4 (DLL4))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLL4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects human Delta-like ligand 4 (DLL4), also known as Delta-4.

Purification: Purified

Immunogen: Synthetic peptide corresponding to the internal region of human DLL4.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on paraffin sections: 5 - 15g/mL. ELISA: 1: 3000 - 1: 15000. Western Blot: 1: 300 - 1: 1500, detects a band of approximately 74 kDa in pancreatic celllysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0mg/mL

Buffer: Phosphate buffered saline pH 7.2 containing 0.09 % Sodium Azide (NaN3)

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-DLL4 antibody (ABIN318838)

Wang, Lu, Zhang, Du, Tang, Wang, Lu, Hu: "GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway." in: Mediators of inflammation, Vol. 2016, pp. 2853040, (2017) (PubMed).

Li, Yan, Wang, Jing, Li, Liu, Yu, Fan: "Effects of canonical NF-κB signaling pathway on the proliferation and odonto/osteogenic differentiation of human stem cells from apical papilla." in: BioMed research international, Vol. 2014, pp. 319651, (2014) (PubMed).

Villaamil, Gallego, Valladares-Ayerbes, Caínzos, Aparicio: "Multiple biomarker tissue arrays: A computational approach to identifying protein-protein interactions in the EGFR/ERK signalling pathway." in: Journal of molecular signaling, Vol. 7, Issue 1, pp. 14, (2012) (PubMed).

González-Mariscal, Garay, Quirós: "Identification of claudins by western blot and immunofluorescence in different cell lines and tissues." in: Methods in molecular biology (Clifton, N.J.), Vol. 762, pp. 213-31, (2011) (PubMed).

Target Details for DLL4

Target: DLL4 (delta-Like 4 (DLL4))

Alternative Name: delta-Like Protein 4 / DLL4 (DLL4 Products)

Synonyms: hdelta2 antibody, Delta4 antibody, si:ch211-244p18.5 antibody, zgc:136596 antibody, delta like canonical Notch ligand 4 antibody, delta-like 4 (Drosophila) antibody, DLL4 antibody, Dll4 antibody, dll4 antibody

Background: DLL4 is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the Notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumour vessels of clear-cell renal tumours and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumour growth, with no apparent toxicity.Synonyms: Delta 4, Drosophila Delta homolog 4

Gene ID: 54567

NCBI Accession: NP_061947

UniProt: Q9NR61

Pathways: Notch Signaling