DLL4
Reactivity: Human
FACS, Func
Host: Mouse
Monoclonal
MHD4-46
unconjugated
Application Notes
Immunohistochemistry on paraffin sections: 5 - 15g/mL. ELISA: 1: 3000 - 1: 15000. Western Blot: 1: 300 - 1: 1500, detects a band of approximately 74 kDa in pancreatic celllysates. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Wang, Lu, Zhang, Du, Tang, Wang, Lu, Hu: "GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway." in: Mediators of inflammation, Vol. 2016, pp. 2853040, (2017) (PubMed).
Li, Yan, Wang, Jing, Li, Liu, Yu, Fan: "Effects of canonical NF-κB signaling pathway on the proliferation and odonto/osteogenic differentiation of human stem cells from apical papilla." in: BioMed research international, Vol. 2014, pp. 319651, (2014) (PubMed).
Villaamil, Gallego, Valladares-Ayerbes, Caínzos, Aparicio: "Multiple biomarker tissue arrays: A computational approach to identifying protein-protein interactions in the EGFR/ERK signalling pathway." in: Journal of molecular signaling, Vol. 7, Issue 1, pp. 14, (2012) (PubMed).
González-Mariscal, Garay, Quirós: "Identification of claudins by western blot and immunofluorescence in different cell lines and tissues." in: Methods in molecular biology (Clifton, N.J.), Vol. 762, pp. 213-31, (2011) (PubMed).
DLL4 is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the Notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumour vessels of clear-cell renal tumours and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumour growth, with no apparent toxicity.Synonyms: Delta 4, Drosophila Delta homolog 4